Particle separation device

Abstract

[Problem] To provide a particle separation device which is easy to operate and whereby particles can be appropriately separated. [Solution] This device has: a syringe 2; a barrel 41 into which a sample liquid S including target particles is injected; a barrel 51 into which a buffer liquid B is injected; a branching tube 3 provided with a first conduit 31 connected to a discharge port 21a of the syringe 2, a second conduit 32 as one fork branched from the first conduit 31, connected to an injection port of the barrel 41, and a third conduit 33 as the other fork branched from the first conduit 31, connected to an injection port of the barrel 51; a first unidirectional valve 31a for backflow prevention, for opening when a syringe side thereof has a positive pressure and closing when the syringe side thereof has negative pressure, the first unidirectional valve 31a intervening partway along the first conduit opening; and a DLD microchannel chip 6 provided with a DLD microchannel structure.

Description

technical field [0001] The present invention relates to a particle separation device for separating target particles from a liquid containing the target particles. Background technique [0002] It is known that tumor cells are detached from the primary tumor tissue or metastatic tumor tissue of the cancer and infiltrated into the blood. Such tumor cells are called Circulating Tumor Cells (CTC: Circulating Tumor Cells), and it is expected that by measuring the number of circulating tumor cells in blood in peripheral blood, it is possible to grasp the development status of cancer and the effect of treatment ( prognosis), or information on the diagnosis of recurrence, early detection of metastasis, etc. [0003] As a method for measuring the number of circulating tumor cells in blood in peripheral blood, there is known, for example, a method described in Patent Document 1 in which cells in a cell sample solution are stained with a fluorescently labeled antibody , make the cel...

AI Technical Summary

Problems solved by technology

[0005] However, in the separation device described in Non-Patent Document 2, since the sample solution injection system and the buffer solution injection system are independent of each other, and syringes and tubes are provided for each, it is necessary to separate the syringe filled with the sample solution and the tube injected with the buffer solution. Simultaneous operation of the same amount of the syringe (push the plunger), and the need to use both hands in the case of a single operation, etc. These operations are cumbersome, and if both are not handled carefully and equally, they will be present in the sample solution and buffer solution In case of large flow differences, proper separation may not be possible

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