SNP marker linked to branch angles of pedicels at bottom parts of cauliflower balls and method and application of SNP marker

A branching angle, cauliflower flower technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of long cycle and low efficiency

Active Publication Date: 2020-10-20
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a SNP marker linked to the branching angle of the pedicel at the bottom of the cauliflower curd, to solve the problem of long period...

Method used

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  • SNP marker linked to branch angles of pedicels at bottom parts of cauliflower balls and method and application of SNP marker
  • SNP marker linked to branch angles of pedicels at bottom parts of cauliflower balls and method and application of SNP marker

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Experimental program
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Embodiment 1

[0059] A method for rapid identification of pedicel branching angle traits at the bottom of a cauliflower curd, the method comprising:

[0060] (1) Extraction of seedling genomic DNA

[0061] The seeds of the cauliflower germplasm material were sown in the plug trays, and the seedlings were raised by conventional methods, and the individual plants were numbered; the tender leaves of each individual plant were taken to extract genomic DNA according to the conventional CTAB (hexadecyltrimethylammonium bromide, hexadecyltrimethylammonium bromide) method. , -20 ℃ storage, spare.

[0062] (2) PCR amplification

[0063] PCR amplification was performed with the genomic DNA of each seedling as the template and ZAAS.SA01 as the primer pair. The PCR system adopts the KASP basic reaction system of the 96-well module: the total volume is 10 μL, including 1 μL DNA template (50 ng / μL), 5 μL KASPmastermix (2×), 0.14 μL primer pair mix, 4 μL ddH 2 O.

[0064] (3) Use LC480softwarev1.5.1 t...

Embodiment 2

[0066] A selected cauliflower variety 'Baiyu 80' (Taiwan Hefeng Seedling Co., Ltd. hybrid) and inbred line '4306-1' (Taiwan Qingnong Seedling Co., Ltd. hybrid 'Qingnong 90 days' inbred F 8 A molecular marker-assisted selection method for the branching angle of the pedicel at the bottom of the curd in the progeny of the hybrid progeny, including:

[0067] (1) The seeds of 'Baiyu 80' and '4306-1' were sown, nursed, and planted in plastic greenhouses; '4306-1' was used as the male parent, and 'Baiyu 80' was used as the female parent to carry out artificial budding hybridization at the flowering stage. After the seed pods are mature, the seeds are collected, and the seeds are planted in a plastic greenhouse the following year. When flowering, artificial pollination is performed and the seeds are harvested.

[0068] (2) The seeds harvested in step (1) were sown in plug trays, and seedlings were raised by conventional methods, and numbered by individual plants; genomic DNA was extra...

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Abstract

The present invention discloses an SNP marker linked to branch angles of pedicels at bottom parts of cauliflower balls and a method and an application of the SNP marker. The SNP marker has any one ofnucleotide sequences shown as SEQ ID NO1, SEQ ID NO2, SEQ ID NO3 and SEQ ID NO4. The method uses the pedicel branch angle trait linking molecular mark to develop KASP primers suitable for high-throughput detection, has advantages of strong timeliness and suitable high-throughput detection, can identify germplasm resources or breeding materials, can greatly improve breeding efficiency, reduces blindness in breeding selection, and is a powerful tool to speed up process of flower ball shape breeding improvement.

Description

technical field [0001] The invention relates to a SNP marker, in particular to a SNP marker linked with the branching angle of the pedicel at the bottom of a cauliflower curd, a method and application thereof. Background technique [0002] The shape of the cauliflower ball has a flat bottom and a slanted bottom. Flat-bottomed curds are generally firmer and have whiter pedicels, while oblique-bottomed curds are generally looser and greener because they are more exposed to sunlight. At present, the market demand for green terrier is increasing day by day. Therefore, it is one of the important directions of cauliflower breeding to make the pedicels greener by breeding cauliflower varieties with oblique bottom curds. [0003] refer to figure 1 (SA means angle in the figure), the most important indicator to distinguish flat-bottomed and oblique-bottomed curds is the branching angle of the pedicels at the bottom of the curds. The branching angle is small. The branching angle ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵振卿顾宏辉盛小光王建升虞慧芳沈钰森
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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