Lung cancer gas marker in expiration and application of lung cancer gas marker in lung cancer screening

A technology for biomarkers and lung cancer, applied in measuring devices, instruments, and material analysis through electromagnetic means, can solve problems such as unstandardized sampling methods, high costs, and inability to identify early pulmonary nodules

Pending Publication Date: 2020-10-20
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used clinical screening method is low-dose computed tomography (LDCT), but this technology still cannot identify whether early pulmonary nodules are malignant or benign
Moreover, the technology is expensive and not suitable for large-scale screening
[0003] Breath detection technology has been proved by a large number of studies to be a potential lung cancer screening method, but a unified and mutually verifiable breath marker for lung cancer has not yet been found. Existing speculations believe that the main reason is that the sampling method is not standardized, The markers have not been verified by multi-center, etc.

Method used

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  • Lung cancer gas marker in expiration and application of lung cancer gas marker in lung cancer screening
  • Lung cancer gas marker in expiration and application of lung cancer gas marker in lung cancer screening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Screening of breath biomarkers associated with lung cancer

[0040] (1) Subject breath sample collection

[0041] The invention screens out the early lung cancer biomarkers in the breath by analyzing the breath of early lung cancer patients and normal people. The breath samples came from three experimental centers: 22 lung cancer patients and 90 normal people in the first experimental center, 27 lung cancer patients and 88 normal people in the second experimental center, and 29 lung cancer patients and 111 normal people in the third experimental center. A normal person, lung cancer patients use tissue biopsy, computed tomography (CT) scanning or puncture three methods in the diagnosis process. All normal controls were accompanying family members in the same ward as the patients. Informed consent was obtained from the patients for the breath test, and all were approved by the organizational ethics committee. The breath sample collection adopts the method of ...

Embodiment 2

[0046] Embodiment 2 Lung cancer cells release acetaldehyde compared with normal lung epithelial cells

[0047] (1) Cell culture

[0048] Common lung cancer cells squamous cell carcinoma cells CALU-1, adenocarcinoma cells SPC-A1 and normal lung epithelial cells BEAS-2B were selected as detection objects. The model of the petri dish is RPMI-1640, add 10% bovine fetal serum and 100U / mL double antibody, at 37°C and 5% CO 2 Cultivate under the condition of , after the cells grow well, continue to cultivate for 24 hours after changing the medium, and use it for detection.

[0049] (2) Testing instrument

[0050]Gas chromatography-mass spectrometry was selected as the detection instrument. The chromatographic column model was TG-624SiIMS (30m×0.32mm×1.8μm). 1min. The temperature of the injection port, transfer line and ion source were all 200°C.

[0051] (3) Sampling method

[0052] Cell culture dish headspace gas was enriched for 40 min using 75 μm Carboxen / PDMS solid-phase mi...

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Abstract

The invention discloses a biomarker for screening early lung cancer. The biomarker is acetaldehyde in expiration of a human body. It is found for the first time that the content of acetaldehyde in exhaled air of a lung cancer patient is reduced, meanwhile, it is verified through in-vitro cells that acetaldehyde released by lung cancer cells is far lower than that released by normal lung epithelialcells, and it is proved that acetaldehyde can serve as a biomarker to be applied to lung cancer screening.

Description

technical field [0001] The invention relates to the technical field of detection of lung cancer, in particular to the application of acetaldehyde in exhaled breath in rapid non-invasive screening of lung cancer. Background technique [0002] Lung cancer is the most diagnosed type of cancer worldwide each year and causes the most annual deaths of any cancer. According to the survey, the five-year survival rate of lung cancer patients will drop from 45% in stage I to 1% in stage IV. However, since there are no obvious symptoms in the early stage of lung cancer, most patients are already in stage IV when they are discovered, resulting in a low overall survival rate. . Therefore, a lung cancer screening technique is urgently needed. The commonly used clinical screening method is low-dose computed tomography (LDCT), but this technology still cannot identify whether early pulmonary nodules are malignant or benign. Moreover, the technique is expensive and not suitable for large-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N27/62G01N21/25G01N33/574
CPCG01N30/02G01N27/62G01N21/25G01N33/57423
Inventor 邹雪沈成银黄超群储焰南
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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