Plasmid extraction device

Abstract

The invention relates to the technical field of molecular biology, in particular to a plasmid extraction device. The device comprises a cracking container, a precipitation container, an eluate container, a collection container and a chromatographic column, the cracking container is communicated with the precipitation container through a first connecting pipe, the precipitation container is communicated with the chromatographic column through a second connecting pipe, the eluate container is communicated with the chromatographic column through a third connecting pipe, valves are arranged on thefirst connecting pipe, the second connecting pipe and the third connecting pipe, the collection container is arranged below the chromatographic column, and the chromatographic column is connected toa vibration mechanism. By arranging the vibration mechanism, the chromatographic column can generate slight vibration during separation, so that solid-liquid separation in the chromatographic column is accelerated, and the extraction speed of plasmids is increased.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a plasmid extraction device. Background technique [0002] Plasmid extraction usually refers to the extraction of DNA / RNA in the solution, which requires the use of an injection-type nucleic acid adsorption column to complete the separation of DNA / RNA, usually at the bottom of the injection-type nucleic acid adsorption column to allow the waste liquid in the solution to flow through However, the membrane that cannot pass through the DNA / RNA is used to intercept and extract the DNA / RNA, and then dissolve them through a liquid that can dissolve the DNA / RNA so that it can pass through the membrane to remove the DNA / RNA from the membrane. [0003] The Chinese utility model patent with the publication number CN207091397U discloses a plasmid extraction device, including a chromatography column, a filter funnel, a filtrate receiving part, a filtrate storage part, a waste liqui...

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Problems solved by technology

[0003] The Chinese utility model patent with the publication number CN207091397U discloses a plasmid extraction device, including a chromatography column, a filter funnel, a filtrate receiving part, a filtrate storage part, a waste liquid collection part, an eluent storage part and a plasmid collection part, and the filter funnel , the filtrate receiving part, the filtrate storage part, the chromatographic column and the waste liquid collection part are in fluid communication in sequence, a first peristaltic pump and a first stop valve are arranged on the flow path between the filtrate storage part and the chromatographic column, and the chromatographic column and the chromatographic column A second stop valve is set on the flow path between the waste liquid collection parts, the eluent storage part, the chromatographic column and the plasmid collection part are in fluid communication in sequence, and a second stop valve is set on the flow path between the eluent storage part and the chromatographic column. The peristaltic pump and the third stop valve, and the fourth stop valve are installed on the flow path between the chromatography column and the plasmid collection part. The disadvantage is that the chromatography column needs to be temporarily fixed in a position, and the separation speed is slow

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