Antibody chip kit for quantitatively detecting multiple tumor markers
A tumor marker and quantitative detection technology is applied in the field of antibody chip kits for quantitative detection of multiple tumor markers, which can solve the problems of complex material processing and large consumption.
Active Publication Date: 2020-10-23
山东圣剑医学研究有限公司
View PDF12 Cites 2 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0003] Existing tumor markers are generally detected by flow cytometry. At present, there are many researchers who detect tumors based on fluorescence-encoded microspheres and other technologies. markers, but the above methods need to set up corresponding detection reagents for different tumor markers to perform quantitative detection, the material processing is complicated, and the amount of use is large
One test requires quantitative detection reagents for all tumor markers, but in actual testing, at most one or two markers can be detected
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0030] An antibody chip kit for the quantitative detection of multiple tumor markers, including a microfluidic chip, an ultrasonic exciter, a magnetic controller, qualitative nano-microspheres of different particle sizes, quantitative magnetic microspheres, thiol luminescent group reagents, multiple Antibodies and fluorescence detectors for tumor markers; each qualitative nanosphere surface can be modified with an antibody for a tumor marker, and the surface of qualitative nanospheres can be modified with thiol luminescent groups; the surface of quantitative magnetic nanospheres can be simultaneously modified A variety of tumor marker antibodies, and the quantitative magnetic particles themselves have fluorescence or characteristic light absorption peaks;
[0031] The microfluidic chip includes a sample loading area 11, a reaction area 12, a transport area 13 and a detection area; the sample loading area 11 includes a reagent loading area 11 and a sample loading area 11, and th...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to an antibody chip kit for quantitatively detecting multiple tumor markers. Qualitative nano microspheres and quantitative magnetic microspheres are combined, so that qualitative and quantitative detection can be carried out at the same time at a time; ultrasonic resonance driving and magnetic field control are combined, qualitative detection and quantitative detection can be distinguished, and due to the fact that the use amount of qualitative detection is low, qualitative detection does not affect quantitative detection; operation is easy, and stability is good; qualitative detection is carried out by using qualitative microspheres with different particle sizes, so that the accuracy is good, and the anti-interference capability is strong; each tumor marker does notneed to be independently treated by using quantitative detection, so that the applicability is good; and in addition, ultrasonic and magnetic field control structures can be detached and used repeatedly, and thus the economical efficiency is good.
Description
technical field [0001] The invention relates to the field of medical detection, in particular to an antibody chip kit for quantitatively detecting multiple tumor markers and a manufacturing method thereof. Background technique [0002] Tumor markers are a class of substances that are synthesized and released by tumor cells themselves, or increased by the body in response to tumor cells, and its main components are proteins, sugars, etc. Tumor markers exist in blood, body fluids, and tissues, and can be detected by methods such as biochemistry, immunology, and genomics, and can be used for early diagnosis of tumors, prognosis prediction, and efficacy evaluation. The abnormal increase of tumor markers can indicate the occurrence of tumor, but the abnormal increase of tumor markers can also occur in non-neoplastic diseases. [0003] The existing detection of tumor markers generally adopts the method of flow cytometry. At present, many researchers detect tumor markers based on ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/574G01N33/543B01L3/00
CPCG01N33/582G01N33/574G01N33/54346G01N33/54326B01L3/5027B01L3/502707B01L2200/10B01L2300/0887
Inventor 赵曙华陈婷珺李慧新盖戈王佳佳王芬
Owner 山东圣剑医学研究有限公司