Reagent and kit for detecting two leishmania through isothermal amplification and application of reagent and kit

Abstract

The invention discloses a reagent, kit and detection method for detecting two leishmania through isothermal amplification. The reagent comprises the following loop-mediated isothermal nucleic acid amplification primer groups for detecting the leishmania dualei: a first outer primer SEQ ID No. 1, a second outer primer SEQ ID No. 2, a first inner primer SEQ ID No. 3, a second inner primer SEQ ID No.4 and a first loop primer SEQ ID No. 5; and the loop-mediated isothermal nucleic acid amplification primer group for detecting the infant leishmania: a third outer primer SEQ ID No. 6, a fourth outerprimer SEQ ID No. 7, a third inner primer SEQ ID No. 8, a fourth inner primer SEQ ID No. 9, a second loop primer SEQ ID No. 10 and a third loop primer SEQ ID No. 11. The kit comprises the reagent. The reagent and the kit are used for detecting two leishmania, are high in sensitivity, good in repeatability, low in false negative and false positive and high in detection accuracy, and can directly judge a result by observing color changes with naked eyes.

Description

Technical field [0001] This application of molecular biology techniques, and in particular, to a detection of two isothermal amplification Leishmania reagent kit and its application. Background technique [0002] Leishmaniasis is a disease caused by Leishmania, which spread through the bite of some of the lacewing. The disease occurs in three main ways: skin, mucous membranes, skin or visceral. Therefore leishmaniasis has been divided into cutaneous leishmaniasis and visceral leishmaniasis (kala-azar that is). [0003] Kala-azar is the most severe form of leishmaniasis, may be fatal if left untreated. In a few months to several years fever may also occur, spleen and liver damage and anemia after infection, there may be a "post-kala-azar cutaneous leishmaniasis." The disease is the world's second-largest parasitic killer (after malaria), resulting in an estimated deaths worldwide from 20,000 to 30,000 cases per year. [0004] Currently there are two known on the world Leishmania c...

AI Technical Summary

Problems solved by technology

[0008] The purpose of this application is to provide a reagent, kit and application for the detection of two kinds of Leishmania by isothermal amplification, aiming at solving the need for professional operation in the prior art fluorescent PCR detection method, and the high cost and inconvenient detection technical issues

Images