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Extraction method of L-malic acid

An extraction method and malic acid technology, applied in the field of L-malic acid extraction, can solve the problems of low extraction purity, complex process, low yield and the like, and achieve high yield and purity, simplified operation process and high yield. Effect

Pending Publication Date: 2020-11-06
新拓洋生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for extracting L-malic acid to solve the problems of complex process, low extraction purity and low yield in traditional extraction methods

Method used

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  • Extraction method of L-malic acid

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Comparison scheme
Effect test

Embodiment 1

[0027] The extraction method of the L-malic acid of the present embodiment, comprises the following steps:

[0028] 1) Acidification: acidify the L-malic acid fermentation broth with oxalic acid until the pH is 2.0, remove calcium salts, mycelia and fermentation residues by vacuum filtration, and wash the filtered filter cake with deionized water until the pH is 5.0 to ensure that no Malic acid is lost to obtain L-malic acid acidification solution.

[0029] 2) Impurity removal: under the condition of 35°C, put the L-malic acid acidification solution into the TY019 separation resin column, the column flow rate is 50mL / min, and the feed amount is 150g, and the separation solution is collected in sections, and the first section is collected as chromatographic resin The water in the gap is collected and reused; the second section collects the impurity solution and discards it; the third section collects the low-purity L-malic acid solution, which is stored for the next separation;...

Embodiment 2

[0033] The extraction method of the L-malic acid of the present embodiment, comprises the following steps:

[0034] 1) Acidification: acidify the L-malic acid fermentation broth with oxalic acid until the pH is 1.8, vacuum filter to remove calcium salts, mycelia and fermentation residues, and wash the filtered filter cake with deionized water until the pH is 5.0 to ensure that no Malic acid is lost to obtain L-malic acid acidification solution.

[0035]2) Impurity removal: under the condition of 35°C, put the L-malic acid acidification solution into the TY019 separation resin column, the column flow rate is 80mL / min, the feed amount is 100g, and the separation solution is collected in sections, and the first section is collected as chromatographic resin The water in the gap is collected and reused; the second section collects the impurity solution and discards it; the third section collects the low-purity L-malic acid solution, which is stored for the next separation; the four...

Embodiment 3

[0039] The extraction method of the L-malic acid of the present embodiment, comprises the following steps:

[0040] 1) Acidification: acidify the L-malic acid fermentation broth with oxalic acid until the pH is 2.5, remove calcium salts, mycelia and fermentation residues by vacuum filtration, and wash the filtered filter cake with deionized water until the pH is 5.0 to ensure that no Malic acid is lost to obtain L-malic acid acidification solution.

[0041] 2) Impurity removal: under the condition of 45°C, put L-malic acid acidified liquid into TY019 separation resin column, the flow rate of the column is 25mL / min, the feeding amount is 200g, and the separation liquid is collected in sections, and the first section is collected as chromatographic resin The water in the gap is collected and reused; the second section collects the impurity solution and discards it; the third section collects the low-purity L-malic acid solution, which is stored for the next separation; the fourt...

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Abstract

The invention relates to an extraction method of L-malic acid, and belongs to the technical field of downstream extraction of organic acid biological fermentation. The extraction method of the L-malicacid comprises the following steps: 1) adding oxalic acid into an L-malic acid fermentation liquid, acidifying, and filtering to obtain a filtrate, namely an L-malic acid acidified liquid; 2) separating and removing impurities from the L-malic acid acidified liquid through a TY019 resin to obtain an L-malic acid separated liquid; 3) concentrating the L-malic acid separated liquid in the step 2),and crystallizing to obtain an L-malic acid wet product; and 4) placing the L-malic acid wet product in a vacuum drying oven, and performing vacuum gradient drying to obtain an L-malic acid finished product. According to the invention, the TY019 resin is used for separating the L-malic acid acidified liquid, so that the impurity removal effect is good, and the purity of the separated liquid is high; and compared with a conventional L-malic acid extraction method, the method of the invention has the advantages that the yield and purity of the L-malic acid product are high, the operation processis greatly simplified, and the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of downstream extraction of organic acid biological fermentation, and in particular relates to an extraction method of L-malic acid. Background technique [0002] As a food additive and functional food with excellent performance, L-malic acid is widely used in the fields of food, health care products, medical treatment and cosmetics. L-malic acid is generally used as a sour agent, pH regulator, antioxidant synergist, and is widely used in wine, beverages, candies, jams and jellies, margarine, soy sauce, vinegar, pickles and other foods. L-malic acid has the functions of anti-fatigue and protecting the liver, kidney and heart. It can also be used in health care products and the pharmaceutical industry, such as in pharmaceutical preparations, tablets, syrups, amino acid solutions, and insect repellents. It can treat liver diseases, Anemia, low immunity, uremia, high blood pressure, liver failure and other dis...

Claims

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Application Information

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IPC IPC(8): C07C59/245C07C51/48C07C51/47C07C51/43
CPCC07C51/48C07C51/47C07C51/43C07C59/245
Inventor 高莉曹晓伟曹琳青崔凤霞周俊俊秦天苍郭金权张建国史立军陈黎张抗杨拓龚舒胡翠芳宋莹莹李永生
Owner 新拓洋生物工程有限公司
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