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Canine coronavirus fluorescent aptamer test strip

A coronavirus, aptamer technology, applied in the direction of fluorescence/phosphorescence, material analysis by optical means, measurement device, etc., can solve problems such as difficulty in diagnosis, and achieve the effect of accurate detection

Pending Publication Date: 2020-11-17
苏州阿科索生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical symptoms and epidemiology of the disease are similar to rotavirus infection, and it is often mixed with rotavirus, canine parvovirus, etc., making diagnosis difficult

Method used

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  • Canine coronavirus fluorescent aptamer test strip
  • Canine coronavirus fluorescent aptamer test strip

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Embodiment Construction

[0022] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0023] Using nucleic acid aptamer fluorescent probes, a fluorescence analysis method for detecting canine coronaviruses can be established based on the principle of fluorescence resonance energy transfer; canine coronaviruses are excited at a wavelength of 375nm and can fluoresce at 430nm, and canine coronaviruses are labeled with After the nucleic acid aptamer of fluorescein is combined, fluorescence resonance energy transfer can occur between the two, and the fluo...

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Abstract

The invention discloses a canine coronavirus fluorescent aptamer test strip, and relates to the technical field of test strips. A canine coronavirus-aptamer is subjected to fluorescence labeling, andthe 5'end of a DNA single strand complementary with the aptamer is labeled with a quenching group; when no canine coronavirus exists in the system, the canine coronavirus-aptamer is combined with theaptamer complementary DNA, so that fluorescence on the aptamer is quenched; when the canine coronavirus is added and combined with the canine coronavirus-aptamer, a canine coronavirus fluorescent aptamer compound is formed, finally, the structure of the aptamer is changed, aptamer complementary DNA single strands are released, and quenched fluorescence is recovered again. The concentration of thecanine coronavirus can be quantitatively detected by using a fluorescence quantitative instrument through the change of fluorescence.

Description

technical field [0001] The invention belongs to the technical field of test strips, in particular to a canine coronavirus fluorescent aptamer test strip. Background technique [0002] Canine coronavirus (CCV) belongs to Nidoviridae, Coronaviridae, Coronaviridae, Coronavirus I group. The diameter of CCV virions is 60-200nm, spherical and enveloped. The nucleocapsid is filamentous and helically symmetrical, like other enveloped viruses. The disease mainly occurs in the cold winter, spreads rapidly, and often breaks out in litters within a few days; although the occurrence of the disease has no distinction in breed, age, or sex, when it is popular among dogs, it usually occurs in puppies first, and then spreads to other dogs. Dogs of other ages. [0003] The morbidity and mortality rates of puppies are higher than that of adult dogs. The symptoms of puppies are aggravated, vomiting and diarrhea are the main symptoms of the disease, and the symptoms of adult dogs are mild. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/569G01N21/64
CPCG01N33/533G01N33/56983G01N21/6428G01N2021/6432
Inventor 观文娜谢尚晋欧荣华孙琳张凤艳
Owner 苏州阿科索生物科技有限公司
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