A method for constructing a PDX model of hepatocellular carcinoma

A hepatocellular carcinoma model technology, applied in the field of hepatocellular carcinoma PDX model construction, can solve the problems of low success rate of hepatocellular carcinoma PDX model modeling, high tumor formation rate, etc.

Active Publication Date: 2022-03-08
ZHEJIANG UNIV
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Problems solved by technology

[0013] The present invention aims at the problem of low modeling success rate of the PDX model of hepatocellular carcinoma in the prior art, and provides a composition for judging the tumor formation rate when the PDX model of hepatocellular carcinoma is constructed for the purpose of non-medical diagnosis, and A method for constructing a PDX model of hepatocellular carcinoma. By predicting the tumor formation rate of the PDX model of hepatocellular carcinoma, samples with a high tumor formation rate are selected for modeling, and samples with a low tumor formation rate or with no possibility of tumor formation can be discarded modeling

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  • A method for constructing a PDX model of hepatocellular carcinoma
  • A method for constructing a PDX model of hepatocellular carcinoma
  • A method for constructing a PDX model of hepatocellular carcinoma

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Embodiment 1

[0041] Liver cancer PDX modeling and tumor formation prediction analysis method:

[0042] Patients signed the informed consent before the operation;

[0043] a. Acquisition of tumor tissue: fresh tumor tissue (coded as F0) from patients with hepatocellular carcinoma was obtained under aseptic conditions in the operating room, placed in DMEM / F12 medium, and transported to the animal room on ice within 2 hours;

[0044]b. Divide the tumor tissue into three parts in the ultra-clean bench: the first part is to cut the tumor tissue block into small pieces with a diameter of about 2 mm in a sterile environment, and quickly plant them into the immune-deficient mice; the second part is to The tumor tissue blocks were fixed in formalin solution and embedded in paraffin for later molecular pathological detection; the third part was to immediately freeze the tumor tissue blocks with liquid nitrogen for DNA / RNA extraction;

[0045] c. Construction of PDX model: The small pieces of tumor ...

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Abstract

The invention discloses a composition for judging the tumor formation rate when constructing a PDX model of hepatocellular carcinoma for the purpose of non-disease treatment, and a method for constructing a PDX model of hepatocellular carcinoma using the composition. The composition of the present invention for determining the tumor formation rate of hepatocellular carcinoma PDX model construction includes GPC3 and Ki67 in hepatocellular carcinoma tumor tissue samples, and the tumor formation rate of PDX modeling can be judged by detecting these two proteins. The method for predicting the tumor formation of a PDX model of hepatocellular carcinoma according to the present invention is to predict whether the tumor formation of the PDX model is early or not by interpreting the immunohistochemical results of GPC3 and Ki67 in tumor tissues of patients with hepatocellular carcinoma. Early prediction that the PDX model will not form tumors can rule out the construction of PDX models as soon as possible.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing a PDX model of hepatocellular carcinoma. Background technique [0002] PDX (Patient-Derived Xenograft) patient-derived tumor xenograft model refers to planting fresh tumor tissue from patients into immunodeficient mice to achieve growth. The PDX model is characterized by retaining the morphological and structural features, genetic features, and molecular features of the patient's tumor to the greatest extent, which can provide an important platform for studying tumor biology, finding markers, and screening drug targets. PDX models can accurately reflect the efficacy of anti-tumor drugs, and provide important medication suggestions and guidance for individualized precision treatment of clinical cancer patients. my country is a country with a high incidence of liver cancer, of which 90% of liver cancer is hepatocellular carcinoma, which ranks first in the mal...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574A01K67/027
CPCG01N33/57484G01N33/57438A01K67/0271A01K2207/12A01K2227/105A01K2267/0331
Inventor 卓建勇鲁迪王建国连正星张佳丽李慧慧岑贝妮徐骁
Owner ZHEJIANG UNIV
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