Incubator device, cell culture environment control system, and cell culture environment control method

A technology of cell culture and control system, which is applied in the field of incubator devices for cell culture, can solve the problem of low reproducibility, and achieve the effect of reducing the chance of impurity mixing

Pending Publication Date: 2020-11-20
NAT UNIV CORP KUMAMOTO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the judgment of the timing to perform measures such as medium replacement is influenced by the operator's experience, feeling, etc., and the reproducibility is low.

Method used

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  • Incubator device, cell culture environment control system, and cell culture environment control method
  • Incubator device, cell culture environment control system, and cell culture environment control method
  • Incubator device, cell culture environment control system, and cell culture environment control method

Examples

Experimental program
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Effect test

Embodiment 1

[0048] exist figure 1 In , a configuration example of the incubator device 1 (an example of the "incubator device" described in the claims) of the present invention is shown. The incubator device 1 includes a housing 3 (an example of the "cabinet" described in the claims), an LED drive board 5, an LED 7 (an example of the "light source unit" described in the claims), a first aperture (aperture ) substrate 9 , second diaphragm substrate 11 , sensor 13 (an example of the “light measurement unit” described in the claims), sensor drive substrate 15 , support unit 17 , and power supply, control, and communication unit 19 . The LED drive substrate 5 , LED 7 , first diaphragm substrate 9 , second diaphragm substrate 11 , sensor 13 , sensor substrate 15 , and support portion 17 are included in the housing 3 .

[0049] The incubator device 1 has a cell culture space 23 holding a culture medium container 21, and can control the temperature and humidity of the cell culture space 23 unde...

Embodiment 2

[0062] exist figure 2 In , a second embodiment of the incubator device 31 of the present invention is shown. In the incubator device 31 of Embodiment 2, the first diaphragm substrate 9 and the second diaphragm substrate 11 are removed from the incubator device 1 of Embodiment 1, and the light guide member 33 described below ("guide guide" described in the claims) An example of “optical component”) is provided between the lower part of the microwell plate 21 and the sensor 13 . That is, the microplate reader 39 is constituted by the LED driving board 5 , the LED 7 , the sensor 13 , the sensor driving board 15 , and the light guide member 33 .

[0063] The light guide member 33 includes a light guide portion 35 (an example of the “light guide path” described in the claims) made of a transparent light-transmitting silicone resin, and a light shielding member 37 (referred to in the claims) that surrounds the light guide portion. An example of the "shading part" described above)...

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Abstract

The purpose of the present invention is to provide an incubator device or the like capable of performing measurement in a manner such as to minimize changes in the state of a medium. This incubator device controls the cell culture environment and is provided with: an airtight case; a light source for irradiating a medium containing inoculated cells with light; a light measurement unit for measuring the intensity of light from the medium; and a light guide member for guiding the light from the medium to the light measurement unit. The light source unit, the light measurement unit and the lightguide member are placed within the case.

Description

technical field [0001] The present invention relates to an incubator device and the like for cell culture, and particularly relates to an incubator device for cell culture capable of observing the culture state of cells. Background technique [0002] In cell culture, it is necessary to adjust the culture environment for proliferating cells. Specifically, the physicochemical environment such as humidity, pH, osmotic pressure, oxygen partial pressure, and carbon dioxide partial pressure, and the physiological environment such as the concentration of hormones and nutrients are adjusted. Such a culture environment is controlled by the medium in addition to the temperature. [0003] That is, the medium supplies nutrients, growth factors, and hormones required for cell growth, and controlling the pH and osmotic pressure of the culture medium is an important regulator in the adjustment of the culture environment. [0004] Most general mammalian cell lines grow well at pH 7.4. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12M1/34C12M1/36
CPCC12M41/26C12M41/48C12M41/14C12M41/06C12M31/10
Inventor 中岛雄太森田金市
Owner NAT UNIV CORP KUMAMOTO UNIV
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