Protein chip for rapidly detecting pleuroperitoneal carcinoma cells and preparation method thereof

Abstract

The invention provides a protein chip for rapid detection of pleuroperitoneal carcinoma cells and a preparation method of the protein chip, and relates to the technical field of biomedicine. Accordingto the protein chip for rapidly detecting the pleuroperitoneal carcinoma cells and the preparation method of the protein chip, the protein chip comprises a glass slide, the glass slide is divided into a plurality of micro-array areas in a square array mode, and carboxyl magnetic beads are attached to the interior of each micro-array area. According to the protein chip for rapid detection of pleuroperitoneal carcinoma cells and a preparation method of the protein chip, carboxyl magnetic beads are attached to a glass slide; meanwhile, the carboxyl magnetic beads and the colloidal gold labeled antibody are subjected to crosslinking; the carboxyl magnetic beads and the colloidal gold labeled antibodies are subjected to cross-linking, and the sample application is performed in each micro-arrayregion, so that the colloidal gold labeled antibodies can be contained in each micro-array region, and the thoracic and ascites cancer cell detection precision can be effectively improved in the useprocess.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a protein chip for rapid detection of pleural effusion cancer cells and a preparation method thereof. Background technique [0002] Pathological diagnosis of cancer cells in pleural and ascites has long been a difficult problem for pathologists, especially when the proliferation of mesothelial cells in pleural and ascites is obvious, they are often very similar in shape to well-differentiated cancer cells, which can be easily confused and cause pathological problems. Diagnosis has brought great difficulties, and even pathologists with rich clinical experience may misdiagnose, which is also a difficult problem in the current international cytopathology community. [0003] For the identification of mesothelial cells and cancer cells in pleural effusion, the most effective solution in the past is to distinguish mesothelial cells and cancer cells by immunohistochemical staining. However, ...

AI Technical Summary

Problems solved by technology

However, in the prior art, since cell smears are not as reproducible as tissue sections, the cell components and distribution on each cell smear are different, so there is no guarantee that there are cancer cells on each smear , so that the detection will not be accurate enough, and at the same time, some existing antibodies cannot be adsorbed on the glass slide for a long time. In use, the antibodies will not be used accurately and the detection accuracy will be reduced.

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