Random optical reconstruction microscopic imaging system

Abstract

The invention discloses a random optical reconstruction microscopic imaging system. The imaging system comprises a light source module which is used for generating exciting light which can excite a dye to generate spontaneous flickering, a laser modulation module which comprises a filter used for modulating the exciting light emitted by the light source module and then outputting the exciting light, a microscopic imaging module which comprises an imaging device is used for imaging when the dye-labeled sample to be detected is excited by the modulated exciting light, and a control module whichis used for controlling the working state of the filter and the exposure state of the imaging device to be synchronous. According to the invention, the control module is used for controlling the working state of the filter to be synchronous with the exposure state of the imaging device, so that the activation of the exciting light to the sample and the imaging of the sample are synchronous, the exciting light is prevented from irradiating the sample for a long time to reduce the activity of the living cell sample and the scintillation duration of the fluorescent dye, and the dynamic process duration of living cells is prolonged.

Description

technical field [0001] The invention relates to the technical field of optical microscopic imaging, in particular to a random optical reconstruction microscopic imaging system. Background technique [0002] Cells and their organelles have important physiological functions in living organisms and have become the focus of scientists' research. However organelles such as mitochondria, endoplasmic reticulum, Golgi apparatus etc. are smaller in size, usually in the nanoscale range. The traditional optical microscope is limited by the optical diffraction limit, and the resolution can only reach about 200nm, while the electron microscope, despite its high resolution, cannot be used for live cell imaging. Aiming at the resolution limit of optical microscopes, scientists have developed several methods that can break through the optical diffraction limit, mainly including stimulated emission depletion microscopy (Stimulated Emission Depletion Microscopy, STED), structured light illum...

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Problems solved by technology

[0004] In view of this, an embodiment of the present invention provides a stochastic optical reconstruction microscopic imaging system to solve the problem of how to prolong the blinking duration of the dye when the stochastic optical reconstruction microscope is used for live cell imaging

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