Method of killing bacteria for non-therapeutic purposes
A bacterium, purpose technology, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve problems such as difficult to cure, easy to infect the blood system, sepsis and pneumonia
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[0015] Embodiment 1 indole, 2-methylindole, 5-methylindole are used as bactericides to kill different types of bacteria
[0016] 1. Activated bacteria: Staphylococcus aureus ATCC 25923 (S.aureus ATCC 25923), absorb 1 μl of 20% glycerol bacteria solution of Staphylococcus aureus ATCC 25923 stored in a -80°C refrigerator, and add it to 1ml LB liquid medium , cultured on a shaker (250rpm) at 37°C to the plateau stage, diluted the obtained bacterial solution 1000 times and inoculated in 50ml LB liquid medium, cultivated to the plateau stage (24h) on a shaker (250rpm) at 37°C, and obtained golden yellow Staphylococcus culture fluid.
[0017] 2. Prepare indole, 2-methylindole and 5-methylindole with dimethyl sulfoxide (DMSO) respectively to have a mother liquor concentration of 1M, then dilute the mother liquor with DMSO to prepare a working solution with a concentration of 200mM, the entire preparation process Should be protected from light.
[0018] 3. Take 37.5ml of plateau sta...
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