Method of killing bacteria for non-therapeutic purposes

A bacterium, purpose technology, applied in botany equipment and methods, biocides, chemicals for biological control, etc., can solve problems such as difficult to cure, easy to infect the blood system, sepsis and pneumonia

Pending Publication Date: 2020-12-04
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This multidrug-resistant Pseudomonas aeruginosa is highly susceptible to infecting the blood system, as well as highly immunocompromised patients such as bone marrow or organ transplants, eventually leading to sepsis and pneumonia, which are difficult to cure

Method used

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  • Method of killing bacteria for non-therapeutic purposes
  • Method of killing bacteria for non-therapeutic purposes
  • Method of killing bacteria for non-therapeutic purposes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 indole, 2-methylindole, 5-methylindole are used as bactericides to kill different types of bacteria

[0016] 1. Activated bacteria: Staphylococcus aureus ATCC 25923 (S.aureus ATCC 25923), absorb 1 μl of 20% glycerol bacteria solution of Staphylococcus aureus ATCC 25923 stored in a -80°C refrigerator, and add it to 1ml LB liquid medium , cultured on a shaker (250rpm) at 37°C to the plateau stage, diluted the obtained bacterial solution 1000 times and inoculated in 50ml LB liquid medium, cultivated to the plateau stage (24h) on a shaker (250rpm) at 37°C, and obtained golden yellow Staphylococcus culture fluid.

[0017] 2. Prepare indole, 2-methylindole and 5-methylindole with dimethyl sulfoxide (DMSO) respectively to have a mother liquor concentration of 1M, then dilute the mother liquor with DMSO to prepare a working solution with a concentration of 200mM, the entire preparation process Should be protected from light.

[0018] 3. Take 37.5ml of plateau sta...

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Abstract

The invention discloses a method of killing bacteria for non-therapeutic purposes, which comprises the following steps: adding an assistant agent into a bacteria solution containing bacteria to be killed to obtain a bacteria solution treatment solution, and carrying out shaking culture on the bacteria solution treatment solution, wherein the , which the assistant agent is indole, 2-methylindole or5-methylindole. By adopting the method disclosed by the invention, various bacteria can be greatly killed, the risk of drug resistance of the bacteria can be effectively reduced, and the better the sterilization effect is along with the prolonging of the treatment time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a non-therapeutic method for killing bacteria. Background technique [0002] Bacterial drug resistance is currently a major public health problem facing the world, and it is also the main cause of chronic infection and infection recurrence after treatment. Bacterial drug resistance is a threat to human life, safety and health, and is an important factor causing loss of human property. In animal-derived bacteria, bacterial drug resistance also poses a major challenge to veterinary public health. Since the discovery of the first antibiotic, penicillin, for more than 70 years, antibiotics have been clinically used as key drugs for the treatment of bacterial infections. However, long-term use of antibiotics induces drug resistance in bacteria, and the abuse of antibiotics also makes drug resistance more and more serious, resulting in the emergence of drug-resistant strains and even "sup...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/38A01P1/00A61L2/16A61L101/44
CPCA01N43/38A61L2/16
Inventor 付新苗孙凤琪
Owner FUJIAN NORMAL UNIV
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