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Multifunctional vacuum ultrafiltration device

A multi-functional and vacuum technology, applied in ultrafiltration, peptide preparation methods, organic chemistry, etc., can solve the problems of inconvenient operation of multiple samples, and achieve the effect of easy widespread use, simple and reasonable structure, and novel design.

Inactive Publication Date: 2020-12-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the extraction of plasmids, centrifuges are required in multiple steps, which is very inconvenient in multi-sample operations, especially for the extraction of large amounts of plasmid DNA.
The current method is inconvenient in the actual operation process of the user

Method used

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  • Multifunctional vacuum ultrafiltration device
  • Multifunctional vacuum ultrafiltration device
  • Multifunctional vacuum ultrafiltration device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: as Figure 1-2 As shown, a multifunctional vacuum ultrafiltration device of the present invention includes a main body 1, one side of the upper surface of the main body 1 is provided with a mounting groove, and a closed box 2 is fixedly installed in the mounting groove, and the closed box The upper end surface of 2 is provided with a number of installation holes 3, and adapters 4 are connected in the installation holes 3, the upper end surface of the main body 1 is fixedly embedded with a digital display window 5 on the side close to the airtight box 2, and close to the digital display A number of control buttons 6 are fixedly installed on the bottom side of the window 5, and a vacuum pump 13 is fixedly installed on the side close to the airtight box 2 inside the main body 1, and the suction end of the vacuum pump 13 is connected with an air guide tube 14. The main body 1 is fixedly mounted with a fixed frame 16 near the top of the vacuum pump 13, and a va...

Embodiment 2

[0028] Example 2: Concentration of protein

[0029] Control the vacuum degree to -72.5±2.5kpa, 30min, ultrafiltration centrifuge tube with a molecular weight cut-off of 50kd; it can realize the concentration of BSA protein solution with different concentrations, and the results are shown in the following table:

[0030] BSA protein solution Solution 1 Solution 2 Protein initial concentration (mg / mL) 7.633 0.905 Protein final concentration (mg / mL) 69.425 21.285 Concentration factor 9 23.5

Embodiment 3

[0031] Embodiment 3: the extraction of plasmid

[0032]According to the instructions of the plasmid extraction kit, it is necessary to use the centrifuge in multiple environments, except for the last two steps (removal and elution of the rinsing solution), all the links that use centrifugation can be completed on the equipment of the present invention. This equipment is especially suitable for the extraction of a large number of plasmids (big extraction). (Plasmids are generally extracted from 30-100mL bacterial culture, and DNA in 6-10mL solution needs to be adsorbed to the membrane of the adsorption column through an extension tube)

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Abstract

The invention discloses a multifunctional vacuum ultrafiltration device which comprises a main machine body, a mounting groove is formed in one side of the upper surface of the main machine body, a closed box is fixedly mounted in the mounting groove, a plurality of mounting holes are formed in the upper end face of the closed box, and adapters are connected in the mounting holes; a digital display window is fixedly embedded in the side, close to the closed box, of the upper end face of the main machine body, a plurality of control buttons are fixedly installed on the bottom side close to thedigital display window, a vacuum pump is fixedly installed on the side, close to the closed box, in the main machine body, and the air suction end of the vacuum pump is connected with an air guide pipe. A fixing frame is fixedly installed at the top end, close to the vacuum pump, of the main machine body and fixedly provided with a vacuum degree controller. The multifunctional vacuum ultrafiltration device is simple and reasonable in structure, novel in design and easy to operate, can be used for ultrafiltration concentration of biomacromolecules such as protein or replacement of a buffer solution, can also be used for a plasmid extraction process, has relatively high applicability and is convenient to widely popularize and use.

Description

technical field [0001] The invention relates to an ultrafiltration device, in particular to a multifunctional vacuum ultrafiltration device. Background technique [0002] The ultrafiltration device is an advanced membrane separation technology. The solvent and various small solutes contained in the feed liquid pass through the filter membrane from the high-pressure feed liquid side to the low-pressure side, thereby obtaining the permeate or ultrafiltrate; its ultrafiltration The micropores of the filter membrane can reach less than 0.01 micron, which can effectively remove particles, colloids, bacteria, heat sources and organic matter in water, while solute molecules with a size larger than the membrane pore diameter are intercepted by the membrane to form a concentrated solution. In the process of scientific research, the ultrafiltration process of centrifuge is usually used in the laboratory to achieve the concentration or exchange of a small amount of protein solution. D...

Claims

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Application Information

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IPC IPC(8): B01D61/18B01D61/14B01D61/20C07K1/34C12M1/12
CPCB01D61/18B01D61/145B01D61/20C07K1/34C12N15/1017
Inventor 李玲张连文张成
Owner NANKAI UNIV