Method for detecting and evaluating sensitization of test object

Abstract

The invention discloses a method for detecting and evaluating sensitization of a test object. The method comprises the following steps: step 1, carrying out early-stage preparation; step 2, preparingand culturing an in-vitro skin tissue sample; step 3, carrying out microdialysis sampling on the in-vitro skin tissue sample; and step 4, carrying out histamine detection on dialysate obtained throughsampling, and evaluating the potential sensitization of a drug. An in-vitro model is constructed by adopting a method of combining skin microdialysis and allergen detection, and in-vitro sensitization detection is carried out by taking in-vitro skin tissues as samples, so that use of animals is avoided, environmental friendliness and harmlessness are achieved, a human in-vitro skin test is more accurate, interference of systematic factors is avoided, and operation is convenient. A plurality of different types of sensitizers can be simultaneously detected, the detection time is short, the effect is more sensitive, and the cost is low; scientific reference is provided for clinical medication, and allergic and anaphylactoid reactions are avoided; the method can be used for scientific research and sampling, and can also be applied clinically to determine the nature of sensitization drugs for patients with allergic diseases.

Description

technical field [0001] The invention relates to an in vitro detection model, in particular to a detection and evaluation method for the sensitization of a test substance. Background technique [0002] Anaphylaxis is usually caused by the immunogenicity of drugs, drug metabolites, drug additives, etc., while anaphylactoid reactions are caused by drugs that stimulate or activate the release of inflammatory mediators. Drug allergic reaction is an idiosyncratic reaction, and it is often difficult to predict its occurrence clinically. Therefore, preclinical drug sensitization evaluation is very important to guide the safe clinical use of drugs. Drug allergy and anaphylactoid reactions are the main unpredictable adverse reactions of drugs, which can lead to symptoms such as skin rash, local edema, asthma, etc., and severe cases can lead to shock or even death. [0003] With the growing call for animal protection in the world, the use of in vitro methods to replace in vivo animal ...

AI Technical Summary

Problems solved by technology

However, clinically, allergic reactions are idiosyncratic reactions with obvious individual differences. In addition, the role of the immune system is extremely complex, and the immune systems of humans and experimental animals are very different. Therefore, it is easy to produce false results by evaluating drug allergy through animal experiments. The currently established in vivo and in vitro sensitization evaluation methods have large differences between the results of the sensitization evaluation of some drugs and the actual clinical results.