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Method for producing volatile chemical and fermentation device

A manufacturing method and technology of chemicals, applied in the directions of biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as pressure rise, danger, reduction of chemical recovery, etc., to reduce losses Effect

Inactive Publication Date: 2020-12-04
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the culture solution is transferred to the storage tank in a state filled with gas containing chemicals, the pressure in the tank will rise and become dangerous, so it is necessary to install a device to reduce the pressure in the tank, such as a vent that releases the gas to the outside of the tank. , Disposal, but if the gas is released out of the tank, the chemicals contained in the gas are also released out of the tank, so the recovery amount of the target chemical is reduced and becomes a loss

Method used

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  • Method for producing volatile chemical and fermentation device
  • Method for producing volatile chemical and fermentation device
  • Method for producing volatile chemical and fermentation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0104] Hereinafter, the present invention will be described in detail with reference to Examples and Comparative Examples. In all the examples and comparative examples, experiments were performed with the ventilation piping, the ventilation port, and the reflux condenser of the culture apparatus always open.

reference example 1

[0105] (Reference example 1) Determination of ethanol

[0106] The ethanol concentration was measured by comparison with a standard product under the gas chromatography conditions shown below.

[0107] Equipment: SHIMADZU GAS CHROMATOGRAPH GC-2010 (SHIMADZU)

[0108] Column: TC-BOND Q 0.25mm*30mm

[0109] Mobile Phase: Helium

[0110] Detection method: FID

[0111] Velocity: 40cm / s

[0112] Temperature: 250°C.

reference example 2

[0113] (Reference example 2) Determination of sugar

[0114] The sugar concentration was quantified by comparison with a standard under the HPLC conditions shown below.

[0115] Equipment: SHIMADZU HPLC system (Shimadzu)

[0116] Column: HILICpak VG-50 4E (Shodex)

[0117] Mobile phase: acetonitrile: water = 3:1

[0118] Detection method: RI detector

[0119] Flow rate mobile phase: 0.6mL / min

[0120] Temperature: 40°C.

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Abstract

A method for producing a volatile chemical, said method comprising, by using a culture device which is provided with a culture tank for culturing a microorganism capable of producing the volatile chemical, a liquid supply line for supplying a liquid culture medium obtained in the culture tank to a storage tank, and the storage tank for storing a solution containing the chemical obtained by culturing the microorganism, culturing the microorganism in the culture tank while holding the solution in the storage tank, wherein a gas phase part in the storage tank is connected to a gas phase part in the culture tank via a ventilation pipe.

Description

technical field [0001] The present invention relates to a manufacturing method and manufacturing apparatus for reducing the loss of volatile chemicals. Background technique [0002] The method of producing various chemicals with microorganisms such as yeast and bacteria using organic compounds in biomass as fermentation raw materials has been put into practical use. The medium extracts the target chemical, or concentrates the medium. In such a case, the culture solution is temporarily collected in a storage tank or the like. [0003] When the chemical to be produced is a volatile chemical, since the volatile chemical evaporates from the culture solution, the culture tank and the storage tank where fermentation is performed are filled with gas containing the chemical. If the culture solution is transferred to the storage tank in a state filled with gas containing chemicals, the pressure in the tank will rise and become dangerous, so it is necessary to install a device to re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/04C12M1/00
CPCC12M21/12C12M33/14C12P7/04C12P7/06C12P7/16C12P7/28C12P7/54Y02E50/10
Inventor 水野宽之耳冢孝须田和美日笠雅史
Owner TORAY IND INC