Unlock instant, AI-driven research and patent intelligence for your innovation.

A h7n9 virus specific recognition antibody p52h12 and detection kit

A P52H12 and detection kit technology, applied in the field of virus diagnosis, can solve problems such as loss of application value of humanized antibodies, loss of antibody affinity and specificity, difficulty in diagnosis of suspected cases, etc., to solve false positives, low cost, highly specific effect

Active Publication Date: 2021-05-18
CANVEST WUHAN BIOTECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of human serum components, it is easy to produce false positive detection, that is, there may still be antibodies against mouse or rabbit antibodies in the serum that has not been infected with the virus, and the serological diagnosis result at this time is a false positive
This will bring great difficulties to the diagnosis of suspected cases without clinical disease
In order to solve this problem, the existing solutions are to change the species of the obtained mouse-derived antibody or rabbit-derived antibody, that is, to humanize it, but in this process, there will be a high probability that the affinity and specificity of the antibody will be reduced. Severe loss of sex, the final humanized antibody also lost its application value

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A h7n9 virus specific recognition antibody p52h12 and detection kit
  • A h7n9 virus specific recognition antibody p52h12 and detection kit
  • A h7n9 virus specific recognition antibody p52h12 and detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1H7

[0025] Example 1 Screening of H7N9 virus specific recognition antibody

[0026] (1) Construction of a phage display library of human Fab antibodies

[0027] The binding experiment was carried out with the convalescent patient serum after infection with H7N9 virus and SH02 HA, 17SF HA, human H5rHA monomer protein (SH02 MONO, 17SF MONO, human H5 MONO), and the experimental results are as follows image 3 As shown, it shows that the serum of the convalescent patients contains antibodies that can bind to H7 or H5 antigen, and BSA is used as a negative control, and there is no non-specific binding. The patient blood with specific binding was selected as the library sample.

[0028] Taking the patient's blood that can specifically bind to the above viral proteins as a sample, separate lymphocytes, extract total RNA, synthesize cDNA by reverse transcription, use cDNA as a template, and use specific primers to amplify the Fab fragment of the antibody. The resulting fragment was liga...

Embodiment 2

[0036] Example 2 Detection of properties of antibody P52H12

[0037] see figure 2 , is the result of affinity determination of P52H12 IgG antibody by ELISA, wherein ● indicates 17SFHA strain (A / Guangdong / 17SF003 / 2016), ■ indicates SH02 HA strain (A / Shanghai / 2 / 2013H7N9), ▲ indicates BSA negative control.

[0038] The results showed that the EC of P52H12 to the SH02 HA and 17SF HA proteins of two strains of H7N9 50 The values ​​were 7.67nM and 34.4nM, respectively, with high affinity and certain cross-activity to the HA protein of H7 subtype; at the same time, the ELISA results showed that the antibody did not bind to the control BSA, and had no non-specificity. This antibody has no neutralizing inhibitory activity.

[0039]

[0040]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an H7N9 virus-specific recognition antibody P52H12 and a detection kit. In the amino acid sequence of the antibody, heavy chain CDR1: GGTFSNYA, CDR2: IIPIFGTA, CDR3: ALPCSSTSCYLVVQGAYYYGMDV, light chain CDR1: QSLLHSNGYNY, CDR2: LGS, CDR3:MQALQTPPT. The antibody can specifically recognize H7N9 influenza virus antigen, has high affinity and high specificity, can effectively solve the common false positive situation in current clinical diagnosis, and provide accurate detection results for the prevention and control of influenza virus.

Description

technical field [0001] The invention relates to the technical field of virus diagnosis, in particular to a specific recognition antibody P52H12 of H7N9 avian influenza virus and a detection kit for the virus. Background technique [0002] Acute respiratory infections are the most common infectious diseases in humans. Most respiratory tract infections are caused by viruses. Common clinical pathogens include adenovirus, influenza virus, parainfluenza virus, respiratory syncytial virus and rhinovirus. Mycoplasma and chlamydia are both respiratory pathogenic bacteria. Because treatment options vary for different bacterial and viral infections, a diagnosis needs to be made as soon as possible after infection. At the same time, due to the high transmissibility and high lethality of the highly pathogenic influenza virus, strict isolation and protection are required. Since general clinical diagnostic methods cannot identify the difference of pathogens, it is necessary to adopt acc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C12N15/13G01N33/569G01N33/577
CPCC07K16/005C07K16/1018C07K2317/24C07K2317/51C07K2317/515C07K2317/565C07K2317/92G01N33/56983G01N33/577G01N2333/11G01N2469/10
Inventor 徐国东幸晓莹刘愈杰
Owner CANVEST WUHAN BIOTECH