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Tissue culture and rapid propagation method of hippophae rhamnoides

A technology for tissue culture rapid propagation and sea buckthorn, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate, easy spread of diseases, low reproduction coefficient of root and tiller, etc., so as to improve the rooting rate. Effect

Inactive Publication Date: 2020-12-18
赵英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Seabuckthorn is dioecious, and it is difficult to maintain the characteristics of excellent varieties in production by seed reproduction; the reproduction coefficient of root tiller reproduction is low
At present, the survival rate of cutting propagation used in production is not high, and it is easy to spread diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The invention provides a tissue culture rapid propagation method of seabuckthorn, comprising the following steps:

[0072] (1) Inoculate the sterilized Seabuckthorn stem section with dormant buds into the starting medium for cultivation, the cultivation conditions are: light intensity: 1200lx, photoperiod: 11h / d, temperature: 26°C;

[0073] (2) Inoculate the vigorously growing stem segments into the rooting medium for cultivation, the cultivation conditions are: light intensity: 1900lx, photoperiod: 16h / d, temperature: 26°C.

[0074] in,

[0075] The formula of the starting medium is: 1 / 3MS+30g / L of sucrose+7g / L of agar+0.3mg / L of IBA.

[0076] The formula of described rooting medium is:

[0077] KNO 3 : 950mg / L;

[0078] NH 4 NO 3 : 825mg / L;

[0079] K H 2 PO 4 : 85mg / L;

[0080] MgSO 4 ·7H 2 O: 185mg / L;

[0081] CaCl 2 2H 2 O: 220mg / L;

[0082] KI: 0.415mg / L;

[0083] h 3 BO 3 : 3.1mg / L;

[0084] MnSO 4 4H 2 O: 11.15mg / L;

[0085] ZnSO 4 ·7H 2...

Embodiment 2

[0102] The invention provides a tissue culture rapid propagation method of seabuckthorn, comprising the following steps:

[0103] (1) Inoculate the sterilized seabuckthorn stem section with dormant buds into the starting medium for cultivation, the cultivation conditions are: light intensity: 1100lx, photoperiod: 12h / d, temperature: 27°C;

[0104] (2) Inoculate the vigorously growing stem segments into the rooting medium for cultivation, the cultivation conditions are: light intensity: 1800 lx, photoperiod: 16h / d, temperature: 27°C.

[0105] in,

[0106] The formula of the starting medium is: 1 / 3MS+30g / L of sucrose+7g / L of agar+0.4mg / L of IBA.

[0107] The formula of described rooting medium is:

[0108] KNO 3 : 950mg / L;

[0109] NH 4 NO 3 : 825mg / L;

[0110] K H 2 PO 4 : 85mg / L;

[0111] MgSO 4 ·7H 2 O: 185mg / L;

[0112] CaCl 2 2H 2 O: 220mg / L;

[0113] KI: 0.415mg / L;

[0114] h 3 BO 3 : 3.1mg / L;

[0115] MnSO 4 4H 2 O: 11.15mg / L;

[0116] ZnSO 4 ·7H ...

Embodiment 3

[0133] The invention provides a tissue culture rapid propagation method of seabuckthorn, comprising the following steps:

[0134] (1) Inoculate the sterilized Seabuckthorn stem section with dormant buds into the starting medium for cultivation, the cultivation conditions are: light intensity: 1300lx, photoperiod: 10h / d, temperature: 25°C;

[0135] (2) Inoculate the vigorously growing stem segments into the rooting medium for cultivation, the cultivation conditions are: light intensity: 2000lx, photoperiod: 16h / d, temperature: 25°C.

[0136] in,

[0137] The formulation of the starting medium is: 1 / 3MS + 30 g / L sucrose + 7 g / L agar + 0.2 mg / L IBA.

[0138] The formula of described rooting medium is:

[0139] KNO 3 : 950mg / L;

[0140] NH 4 NO 3 : 825mg / L;

[0141] K H 2 PO 4 : 85mg / L;

[0142] MgSO 4 ·7H 2 O: 185mg / L;

[0143] CaCl 2 2H 2 O: 220mg / L;

[0144] KI: 0.415mg / L;

[0145] h 3 BO 3 : 3.1mg / L;

[0146] MnSO 4 4H 2 O: 11.15mg / L;

[0147] ZnSO 4 ·7...

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Abstract

The invention provides a tissue culture and rapid propagation method of hippophae rhamnoides. The tissue culture and rapid propagation method comprises the following steps that (1) hippophae rhamnoides stems with dormant buds are inoculated into a starting culture medium for culture under the culture conditions that the illumination intensity is 1100-1300 lx, the photoperiod is 10-12 h / d and the temperature is 26 + / -1 DEG C; and (2) strongly-growing stem segments are inoculated into a rooting culture medium for culture under the culture conditions that the illumination intensity is 1800-2000 lx, the photoperiod is 16h / d and the temperature is 26 + / -1 DEG C; According to the tissue culture and rapid propagation method of the hippophae rhamnoides, the rooting rate is greatly increased by optimizing the component content of an MS culture medium.

Description

technical field [0001] The invention relates to a tissue culture and rapid propagation method of seabuckthorn. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a perennial deciduous fruit tree, shrub or small tree. Its fruit, peel, leaf, bark and its processed products contain more than 280 kinds of physiologically active substances, which have strong special effects of anti-inflammatory, bactericidal, pain-relieving and promoting tissue regeneration. Many of the ingredients have shown miraculous therapeutic effects in killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing vascular embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity. [0003] Seabuckthorn has luxuriant branches and leaves, well-developed lateral roots, and strong root tillering ability, which can quickly form dense groups of roots and tillers, and can form a large number of root nodules i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 赵英郑新国李宏赵一卓程平孔德智张志刚韩晓燕
Owner 赵英