L plantarum LP45 living bacterium preparation for regulating intestinal flora and application
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A technology of Lactobacillus plantarum and bacteria, applied in the biological field, can solve the problems of decreased survival rate of lactic acid bacteria, dead enzyme protein molecules, microbial cell damage, etc., and achieve the effects of improving humoral immunity, stimulating secretion, and inhibiting growth
Active Publication Date: 2020-12-18
河北一然生物科技股份有限公司
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However, the vacuum freeze-drying process is a process in which the free water in the fermentation broth is lost in a frozen state. The two processes of freezing and drying will cause damage and death of some microbial cells and the passivation of some enzyme protein molecules. If freeze-drying Improper selection of protective agent in the process will reduce the survival rate of lactic acid bacteria
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Embodiment 1
[0041] 1. A preparation method for freeze-dried bacterial powder of Lactobacillus plantarum, comprising:
[0042] 1.1 Test materials: Lactobacillus plantarum LP45 strain, skimmed milk powder, purchased from Wandashan Enterprise Group Dairy Co., Ltd.; trehalose, purchased from Hebei Baiyou Biotechnology Co., Ltd.; gelatin, purchased from Guangzhou Jianke Biotechnology Co., Ltd.; Sucrose, commercial grade; glycerin, purchased from Henan Haomiao Chemical Products Co., Ltd.; sodium glutamate, purchased from Jiangxi Baiying Biotechnology Co., Ltd.; L-cysteine, purchased from Jiangsu Xintang Biotechnology Co., Ltd.
[0043] 1.1.1 Preparation of improved MRS agar medium: peptone 10g, beef extract 10g, yeast extract 5g, diammonium hydrogen citrate 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, MgSO4 7H 2 O 0.58g, agar 15g, MnSO 4 ·5H 2 O 0.25g, distilled water 1L.
[0049] 1.1.3 Preparation of freeze-drying protective agent: skimmed milk powder 12g, trehalose 6g, sucrose 5g, glycerin 1.6g, gelatin 2g, sodium glutamate 1g, L-cysteine 0.12g and manganese sulfate 0.3g, distilled water 71.9 g. All the other parts are completely consistent with Example 1.
Embodiment 3
[0051] 1.1.3 Preparation of freeze-drying protective agent: skimmed milk powder 12g, trehalose 6g, sucrose 5g, glycerin 1.6g, 10-hydroxy-2-decenoic acid 0.06g, gelatin 2g, sodium glutamate 1g, L-semi Cystine 0.12g and manganese sulfate 0.3g, distilled water 71.9g. All the other parts are completely consistent with Example 1.
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Abstract
The invention provides an L plantarum living bacterium preparation and an application thereof, and belongs to the technical field of biology. The L plantarum living bacterium preparation comprises freeze-dried L plantarum powder and freeze-dried L plantarum microcapsules, wherein a freeze-drying protective agent used in the preparation process comprises 1-3wt% of glycerol and 0.05-0.15 wt% of 10-hydroxyl-2-decenoic acid, and the mass ratio of the 10-hydroxy-2-decenoic acid to the glycerol is 1: (15-20). The L plantarum viable bacterium preparation provided by the invention has the advantages of high biological activity, capability of enhancing the body immune function and capability of efficiently and durably adjusting intestinal flora balance.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a live Lactobacillus plantarum LP45 preparation for regulating intestinal flora and its application. Background technique [0002] Lactobacillus plantarum (L plantarum) belongs to the Lactobacillus genus in the Lactobacillus family, Gram-positive bacteria, facultative anaerobic, is a probiotic group in the human gastrointestinal tract, metabolism can produce organic, bacteriocin, hydrogen peroxide, Various natural antibacterial substances such as diacetyl can adjust the relationship between the flora, maintain and ensure the optimal combination of the flora and the stability of the combination, prevent the colonization and invasion of pathogenic bacteria, and antagonize pathogenic bacteria and harmful microorganisms The growth and adhesion of toxins can maintain the balance of intestinal flora. However, it is difficult to preserve lactic acid bacteria for a long time. Th...
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