Construction method and application of specific electrochemical sensor for HER2 detection

A construction method and electrochemical technology, applied in the field of electrochemistry, can solve problems such as composite materials that are rarely reported, and achieve the effects of promoting cross-development, expanding applications, and improving accuracy

Active Publication Date: 2020-12-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the reports on this kind of plasmon-enhanced electroactivity are focused on the study of pure Au or Ag nanomaterials, but there are few reports on composite materials.

Method used

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  • Construction method and application of specific electrochemical sensor for HER2 detection
  • Construction method and application of specific electrochemical sensor for HER2 detection
  • Construction method and application of specific electrochemical sensor for HER2 detection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for constructing a specific electrochemical sensor for HER2 detection, specifically comprising the steps of:

[0034] (1) Preparation of AuRu nanoparticles:

[0035] First, PVP (80 mg) and CTAB (60 mg) were dissolved in 15 mL of diethylene glycol solution, and the solution was stirred at room temperature for 20 minutes. It was then placed in a sand bath and heated at 190° C. for 5 minutes with magnetic stirring. Immediately after, 30 mg CTAB and 5 mg RuCl 3 ·3H 2 O was dispersed in 1 mL of diethylene glycol. Then, 20 μL of an ethanol solution of chloroauric acid (0.05 g / mL) was added to the above mixture with stirring. Afterwards, the mixed solution was injected into the previously heated diethylene glycol solution through a flow syringe pump. Subsequently, the solution was heated at a temperature of 190° C. for 5 minutes under magnetic stirring, and the color of the solution was observed to change to dark brown. After the solution was cooled to room temp...

Embodiment 2

[0048] A method for constructing a specific electrochemical sensor for HER2 detection, specifically comprising the steps of:

[0049] (1) Preparation of AuRu nanoparticles:

[0050] First, PVP (88.8 mg) and CTAB (72.9 mg) were dissolved in 20 mL of diethylene glycol solution, and the solution was stirred at room temperature for 30 minutes. It was then placed in a sand bath and heated at 195° C. for 10 minutes with magnetic stirring. Immediately after, 36mg CTAB and 7.8mg RuCl 3 ·3H 2 O was dispersed in 2 mL of diethylene glycol. Then, 24.2 μL of an ethanol solution of chloroauric acid (0.1 g / mL) was added to the above mixture with stirring. Afterwards, the mixed solution was injected into the previously heated diethylene glycol solution through a flow syringe pump. Subsequently, the solution was heated at a temperature of 195° C. for 10 minutes under magnetic stirring, and the color of the solution was observed to change to dark brown. After the solution was cooled to ro...

Embodiment 3

[0063] A method for constructing a specific electrochemical sensor for HER2 detection, specifically comprising the steps of:

[0064] (1) Preparation of AuRu nanoparticles:

[0065] First, PVP (100 mg) and CTAB (80 mg) were dissolved in 25 mL of diethylene glycol solution, and the solution was stirred at room temperature for 40 minutes. It was then placed in a sand bath and heated at 2000 °C for 15 minutes with magnetic stirring. Immediately after, 40mg CTAB and 10mg RuCl 3 ·3H 2 O was dispersed in 3 mL of diethylene glycol. Then, 30 μL of an ethanol solution of chloroauric acid (0.15 g / mL) was added to the above mixture with stirring. Afterwards, the mixed solution was injected into the previously heated diethylene glycol solution through a flow syringe pump. Subsequently, the solution was heated at a temperature of 200° C. for 15 minutes under magnetic stirring, and the color of the solution was observed to change to dark brown. After the solution was cooled to room te...

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Abstract

The invention provides a construction method and application of a specific electrochemical sensor for HER2 detection, and belongs to the technical field of electrochemistry. On the main basis of enhancement of an electrochemical oxidation peak of Ru0 in an AuRu nano material under excitation of light, the electrochemical oxidation peak of Ru0 in AuRu NPs serves as a detection signal, a magnetic electrochemical sensor is designed, and the electrochemical sensor for detecting HER2 is constructed based on the phenomenon; and the specific electrochemical sensor is used for detecting a human epidermal growth factor receptor 2(HER2). The construction method for HER2 detection has the advantages of high accuracy, easy operation and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical technology, and in particular relates to a construction method and application of a specific electrochemical sensor for HER2 detection. Background technique [0002] Since Ru(4d 7 5s 1 ) contains more empty orbitals, which has good catalytic activity and electrical activity. Therefore, Ru nanomaterials are widely used in various fields (such as catalytic hydrogenation, ammonia synthesis, and electrocatalytic oxygen evolution, etc.). And it has recently been found that Ru nanomaterials also have the potential to be electroactive nanobeacons. The electrochemical properties of the beacon can be better improved by combining Ru with other metal nanomaterials to form heterogeneous multimetal nanomaterials. Therefore, there is a need to provide a way to prepare Ru-based nanocomposites with heterostructures to improve the electrical activity of the materials. [0003] Recently, it has been shown that p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 赵媛刘瀚
Owner JIANGNAN UNIV
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