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Molecular marker closely linked with myzus persicae resistance character of cultivar source, primer, application and variety breeding method

A technology of molecular markers and cultivars, applied in the field of molecular biology

Active Publication Date: 2021-01-08
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no commercially promoted aphid-resistant peach tree varieties in the world.

Method used

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  • Molecular marker closely linked with myzus persicae resistance character of cultivar source, primer, application and variety breeding method
  • Molecular marker closely linked with myzus persicae resistance character of cultivar source, primer, application and variety breeding method
  • Molecular marker closely linked with myzus persicae resistance character of cultivar source, primer, application and variety breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Example 1 Analysis of resistance phenotype and genetic law of QMR resistance traits in peach

[0044]The aphid resistance genes Rm1, Rm2 and Rm3 that have been located in peach, the corresponding resistances are all shown as avoidance resistance (figure 2 A) and allergic reactions in the hazardous area (figure 2 B). QMR anti-aphid materials also showed strong repellency under the condition of external aphids. On the second day after receiving aphids, the population density dropped to half, and almost all of them left within 6 days (figure 2 A), and there is no allergic reaction (figure 2 C).

[0045]figure 2 D shows the typical phenotype of aphid-susceptible material, that is, leaf curling caused by aphids implantation and aphids feeding. In order to analyze the genetic law of QMR traits, we conducted resistance identification on 4 F1 segregating populations, and found that the resistance of the offspring of the population showed a significant bimodal pattern, that is, the number of...

Embodiment 2

[0046]Example 2 BSA mixed pool location analysis of QMR resistance to aphid traits

[0047]According to the identification results of the resistance phenotype, 40 individual plants with extreme resistance to aphid and 40 individual plants with extreme susceptibility to aphid were selected to construct a pool of resistant and susceptible genes to conduct BSA location analysis (image 3 ). In order to identify QMR resistance regulatory sites, high-throughput specific fragment sequencing was performed on the two parents and the resistant and susceptible mixed pool. The average reading depth of the resistant parent '09N3-30' was 39 times, and the aphid-susceptible parent'Zhongtaohongyu' The sequencing depth is 38 times, the sequencing depth of the resistant pool and the sensitive pool are 75 times and 61 times, respectively. Among the 880,000 polymorphic markers, 263,800 single nucleotide polymorphisms (SNPs) and 55,900 insertion deletion (Indel) polymorphic markers were screened out, using...

Embodiment 3

[0048]Example 3 Fine positioning analysis of QMR regulatory sites

[0049]Based on the results of BSA mapping, the applicant adopted a genetic fine mapping strategy to continue to narrow the position range of the QMR gene on the chromosome (Figure 4 ).

[0050]Adopt ‘09N-3-30’ x ‘Middle Peach Red Jade’ F1After two years of observation of the individual plants in the separated population, the individual plants with the same phenotype were analyzed. According to the results of BSA positioning, we designed two Indel markers, Indel-21.52 and Indel-29.95 around the positioning interval containing the QMR gene, corresponding to the positions of Pp03:21,523,514 and Pp03:26,958,658 at the two ends of the positioning interval, respectively. Use the above two markers to 288 F1Genotype analysis of individual plants was conducted to find chromosomal recombination, and 41 exchange individual plants were found. For the inner regions of Indel-21.52 and Indel-29.95 two markers, another 12 molecular marke...

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Abstract

The invention relates to a molecular marker closely linked with the myzus persicae resistance character of a cultivar source, a primer, application and a variety breeding method. In the research process of a novel myzus resistance type, namely a QMR myzus resistance material, of a kind of cultivar source, the molecular marker closely linked with the myzus persicae resistance character is found, the nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, and the 72<nd>-91 sites from the 5' end are inserted or deleted. The molecular marker is closely linked with the myzus persicae resistance character, and when the 72<nd>-91 site from the 5' end of the sequence shown in SEQ ID NO.1 is an insertion homozygous genotype, a to-be-detected sample has a myzus sensing character; and when the 72<nd>-91 site from the 5' end of the sequence shown in SEQ ID NO.1 is a deletion homozygous genotype or an insertion / deletion heterozygous genotype, the to-be-detected sample has the myzus resistance character. By detecting the genotype of the molecular marker, whether a plant to be detected has myzus resistance or not can be effectively predicted in the early stage. The invention furtherprovides the primer for detecting the molecular marker and a myzus persicae resistance variety breeding method.

Description

Technical field[0001]The invention belongs to the technical field of molecular biology, and specifically relates to molecular markers, primers, applications, and breed selection methods closely linked to the green peach green aphid resistance traits from cultivated species.Background technique[0002]Peach trees are an important species of economic forest. The cultivation area and output of peach trees in my country rank first in the world. Myzus persicae (Myzus persicae, referred to as Myzus persicae) is the most serious pest of peach trees in spring, which has seriously affected the green and healthy development of the peach industry. Myzus persicae is the most widely distributed piercing-sucking pest in the world. It can feed on more than 200 species of plants. Peach trees are the initial host of the peach aphid. Starting from the budding of the peach tree, it directly harms the new shoots of the peach tree and causes nutrients in the tree body. Loss, curling leaves, stopping new s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 潘磊王志强牛良鲁振华曾文芳崔国朝闫乐乐
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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