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Recombinant novel coronavirus vaccine composition

A technology of vaccine composition and coronavirus, which is applied in the field of recombinant new coronavirus vaccine composition, can solve the problems of weak immunogenicity and achieve the effect of reducing viral load and good immune protection effect

Pending Publication Date: 2021-01-15
SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Generally, when a single protein is used as an antigenic component of a vaccine, its immunogenicity is weak, and an adjuvant is required to enhance the effect of the vaccine

Method used

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  • Recombinant novel coronavirus vaccine composition
  • Recombinant novel coronavirus vaccine composition
  • Recombinant novel coronavirus vaccine composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of vaccine composition

[0022] In order to study the technical effect of the vaccine composition provided by the present invention, the inventors have made the following vaccine composition preparations (0.5ml / dose), each composition contains rS protein, aluminum hydroxide adjuvant and / or CpG 7909. The specific preparation method is as follows: firstly, the CpG 7909 sample is adsorbed on the aluminum hydroxide adjuvant, and the adsorption samples of CpG 7909 / aluminum hydroxide adjuvant (w / w) with different ratios are prepared (here the content of the aluminum hydroxide adjuvant is substantially The above is the content of aluminum element); then add different concentrations of rS protein stock solution to the CpG 7909 / aluminum hydroxide adjuvant sample. After mixing the above formulations thoroughly, store at 2-8°C if not administered immediately. The specific ratio is shown in Table 1.

[0023] Table 1: Components of each vaccine comp...

Embodiment 2

[0025] Example 2: Comparing the immunological evaluation of novel coronavirus vaccines with different adjuvant combinations on the BALB / c mouse model

[0026] 1. Grouping of mice and animal immunization strategy

[0027] Forty-eight SPF-grade female BALB / c mice (6-8 weeks old) were grouped. 8 or 10 mice in each group, 5 groups in total. For group information, see Table 2. The mice were immunized according to the grouping conditions in Table 2. The immunization method was intramuscular injection into the inner thigh of the mice, and the injection volume was 50 μl / dose / mouse (1 / 10 the intended dose for humans). Each mouse was immunized twice with an interval of 3 weeks.

[0028] Table 2: Comparison of grouping of BALB / c mice in experiments with different adjuvant combinations

[0029]

[0030] 2. Vaccine-Induced Antibody Response Detection

[0031] Two weeks after the second immunization, blood was collected from mice in each group, and serum was separated. IgG antibod...

Embodiment 3

[0036] Example 3: Comparing the immunological evaluation of new coronavirus vaccines with different dosage ratios on the BALB / c mouse model

[0037] 1. Grouping of mice and animal immunization strategy

[0038] 140 SPF grade female BALB / c mice (6-8 weeks old) were grouped. 10 in each group, a total of 14 groups. For group information, see Table 3. The mice were immunized according to the grouping conditions in Table 3. The immunization method was intramuscular injection into the inner thigh of the mice, and the injection volume was 50 μl / dose / mouse (1 / 10 the intended dose for humans). Each mouse was immunized twice with an interval of 3 weeks.

[0039] Table 3: Grouping of BALB / c mice in comparative dose ratio experiments

[0040]

[0041] 2. Vaccine-Induced Antibody Response Detection

[0042] Two weeks after the second immunization, blood was collected from mice in each group, and serum was separated. IgG antibody titers against rS protein in serum were detected by...

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Abstract

The invention discloses a recombinant novel coronavirus (SARS-CoV-2) vaccine composition. The vaccine composition comprises a recombinant spike protein with an amino acid sequence shown in SEQ ID NO:1. The vaccine composition further comprises a pharmaceutically acceptable carrier, such as an aluminum adjuvant in combination with a CpG ODN adjuvant. The vaccine composition shows excellent immunogenicity in mice, and can induce an organism to generate high-titer antibody response biased to Th1 and high-level cellular immune response. Animal model experiments show that the vaccine composition can induce high-titer neutralizing antibodies and significantly reduce the viral load inside lung and turbinate tissues.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular, the invention relates to a recombinant novel coronavirus vaccine composition. Background technique [0002] COVID-19 is a respiratory disease caused by a novel coronavirus (SARS-CoV-2). As of October 20, 2020, more than 40 million people have been infected worldwide, and more than 1.1 million people have died from SARS-CoV-2 infection. The global pandemic of COVID-19 has had a huge impact on human health and economic development. Therefore, it is urgent to find a way to curb the spread of the virus. Vaccines are an effective and cost-effective way to eradicate the SARS-CoV-2 virus. According to the World Health Organization (WHO), as many as 42 different types of vaccine candidates have successfully entered clinical trials. [0003] SARS-CoV-2 belongs to subgroup B of the genus Beta and is an enveloped single-stranded positive-sense RNA virus. The virus genus also includes SARS-CoV ...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61P31/14A61P11/00
CPCA61K39/12A61K39/39A61P31/14A61P11/00C12N2770/20034A61K2039/55505A61K2039/55561A61K2300/00Y02A50/30
Inventor 刘海涛宋玉娇李佳黛周晨亮张玲莉刘革
Owner SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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