Method for extracting trilobatin and phlorizin from lithocarpus litseifolius

Abstract

The invention discloses a method for extracting trilobatin and phlorizin from lithocarpus litseifolius. The method comprises the following steps: (1) treating lithocarpus litseifolius leaves to obtainlithocarpus litseifolius leaf powder; (2) mixing the lithocarpus litseifolius leaf powder with an ethanol aqueous solution, and extracting the trilobatin and the phlorizin under an ultrasonic condition; and (3) centrifuging the extracting solution, and taking the supernatant to obtain the trilobatin and the phlorizin. In an extract obtained by the extraction method disclosed by the invention, thecontent of the trilobatin is greater than 162.93 mg / g, the content of the phlorizin is greater than 32.76 mg / g, the antioxidant value of the trilobatin is greater than 53.29%, and the antioxidant value of the phlorizin is greater than 42.90%. According to the extraction method disclosed by the invention, the liquid-to-solid ratio and the ethanol concentration are effectively reduced, the extraction time is shortened, the use amount of an organic solvent is reduced, the extraction efficiency is greatly improved, and besides, the antioxidant values of the trilobatin and the phlorizin are remarkably increased.

Description

technical field

[0001] The invention relates to a method for extracting trilobatin and phlorizin from plants, in particular to a method for extracting trilobatin and phloridin from leaves of Coedeum japonicus and belongs to the field of extraction of trilobatin and phloridin. Background technique

[0002] Lithocarpus litseifolius (Hance) Chun, also known as sweet tea, is a natural plant rich in active ingredients. Among them, flavonoids are one of the main chemical components of sweet tea, mainly including phlorizin and trilobatin, which belong to dihydrochalcones in sweet tea flavonoids, and have high development value. According to reports, trilobatin and phlorizin have the functions of lowering blood sugar, anti-oxidation, anti-cancer, anti-aging, etc., and are safe and non-toxic, and have great application value for health functions.

[0003] So far, there are few research reports on the extraction methods of sweet tea trilobatin and phlorizin. Among them, the Chinese ...

Examples

Embodiment 1

[0031] The extraction of embodiment 1 sweet tea trilobatin and phlorizin of the present invention (small test)

[0032] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 20 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0033] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 162.93±9.321mg / g and 32.76±4.419mg / g respectively; the antioxidant values ​​were 53.29±0.116%, 42.90±0.516%, respe...

Embodiment 2

[0034] The extraction of embodiment 2 sweet tea trilobatin and phlorizin of the present invention (small test)

[0035] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (40%). After ultrasonic extraction at room temperature (25° C.) for 10 min, the supernatant was collected to obtain an extract. The ultrasonic power was 270 W.

[0036] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 136.58±0.02mg / g and 29.31±0.03mg / g respectively.

Embodiment 3

[0037] Embodiment 3 The extraction of sweet tea trilobatin and phlorizin of the present invention (small test)

[0038] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 30 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0039] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 142.51±0.15mg / g and 30.21±0.03mg / g, respectively.

technical field

[0001] The invention relates to a method for extracting trilobatin and phlorizin from plants, in particular to a method for extracting trilobatin and phloridin from leaves of Coedeum japonicus and belongs to the field of extraction of trilobatin and phloridin. Background technique

[0002] Lithocarpus litseifolius (Hance) Chun, also known as sweet tea, is a natural plant rich in active ingredients. Among them, flavonoids are one of the main chemical components of sweet tea, mainly including phlorizin and trilobatin, which belong to dihydrochalcones in sweet tea flavonoids, and have high development value. According to reports, trilobatin and phlorizin have the functions of lowering blood sugar, anti-oxidation, anti-cancer, anti-aging, etc., and are safe and non-toxic, and have great application value for health functions.

[0003] So far, there are few research reports on the extraction methods of sweet tea trilobatin and phlorizin. Among them, the Chinese ...

Examples

Embodiment 1

[0031] The extraction of embodiment 1 sweet tea trilobatin and phlorizin of the present invention (small test)

[0032] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 20 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0033] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 162.93±9.321mg / g and 32.76±4.419mg / g respectively; the antioxidant values ​​were 53.29±0.116%, 42.90±0.516%, respe...

Embodiment 2

[0034] The extraction of embodiment 2 sweet tea trilobatin and phlorizin of the present invention (small test)

[0035] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (40%). After ultrasonic extraction at room temperature (25° C.) for 10 min, the supernatant was collected to obtain an extract. The ultrasonic power was 270 W.

[0036] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 136.58±0.02mg / g and 29.31±0.03mg / g respectively.

Embodiment 3

[0037] Embodiment 3 The extraction of sweet tea trilobatin and phlorizin of the present invention (small test)

[0038] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 30 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0039] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 142.51±0.15mg / g and 30.21±0.03mg / g, respectively.

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