Method for extracting trilobatin and phlorizin from lithocarpus litseifolius

A technology for trilobatin and phloridzin, which is applied in the field of extracting trilobatin and phloridizin, can solve the problems of large consumption of organic solvents, long extraction time, and many extraction times, and achieves increasing extraction amount, shortening extraction time, The effect of reducing the concentration of ethanol

Pending Publication Date: 2021-01-15
CHENGDU AGRI SCI & TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the Chinese patent whose publication number is CN108948104A discloses a heating and reflux process for extracting trilobatin from Kelehin, which has the disadvantages of long production cycle, complex process, and high cost; the Chinese patent whose publication number is CN110143988A discloses A method for extracting trilobatin from sweet tea by adsorption method mainly has the problems of high cost and low purity; the Chinese patent with publication number CN109134557A discloses a method for extracting phlorizin from sweet tea by Soxhlet reflux. The main disadvantages of the method are: many times of extraction, high temperature, and easy loss; the Chinese patent with publication number CN106220693A discloses a method for ultrasonically extracting sweet tea phlorizin, and the main disadvantage of this method is that it takes too long
[0004] In summary, the existing sweet tea trilobatin and phlorizin extraction methods have various defects such as too long extraction time, large consumption of organic solvent, high cost, low yield, low purity, etc.
Therefore, the extraction process of sweet tea trilobatin and phlorizin needs to be further studied and improved at present.

Method used

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  • Method for extracting trilobatin and phlorizin from lithocarpus litseifolius
  • Method for extracting trilobatin and phlorizin from lithocarpus litseifolius
  • Method for extracting trilobatin and phlorizin from lithocarpus litseifolius

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The extraction of embodiment 1 sweet tea trilobatin and phlorizin of the present invention (small test)

[0032] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 20 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0033] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 162.93±9.321mg / g and 32.76±4.419mg / g respectively; the antioxidant values ​​were 53.29±0.116%, 42.90±0.516%, respe...

Embodiment 2

[0034] The extraction of embodiment 2 sweet tea trilobatin and phlorizin of the present invention (small test)

[0035] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (40%). After ultrasonic extraction at room temperature (25° C.) for 10 min, the supernatant was collected to obtain an extract. The ultrasonic power was 270 W.

[0036] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 136.58±0.02mg / g and 29.31±0.03mg / g respectively.

Embodiment 3

[0037] Embodiment 3 The extraction of sweet tea trilobatin and phlorizin of the present invention (small test)

[0038] Take wood ginger leaves Ke Nen leaves (sweet tea), dry at 50°C, grind at 17800rpm for 1min, pass through an 80-mesh sieve to obtain tea powder, seal it in a dry container, and store it at 4°C until use; The sample powder was put into a beaker and mixed with 20 mL ethanol aqueous solution (70%). After ultrasonic extraction at room temperature (25° C.) for 30 min, the supernatant was collected to obtain an extract. The ultrasonic power was 450 W.

[0039] The obtained extract was diluted 200 times, and the obtained diluted sample was used to measure the contents of trilobatin and phlorizin in sweet tea by HPLC, and then the antioxidant activity was measured by DPPH-spiking method. Determination results: the contents of trilobatin and phlorizin were 142.51±0.15mg / g and 30.21±0.03mg / g, respectively.

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Abstract

The invention discloses a method for extracting trilobatin and phlorizin from lithocarpus litseifolius. The method comprises the following steps: (1) treating lithocarpus litseifolius leaves to obtainlithocarpus litseifolius leaf powder; (2) mixing the lithocarpus litseifolius leaf powder with an ethanol aqueous solution, and extracting the trilobatin and the phlorizin under an ultrasonic condition; and (3) centrifuging the extracting solution, and taking the supernatant to obtain the trilobatin and the phlorizin. In an extract obtained by the extraction method disclosed by the invention, thecontent of the trilobatin is greater than 162.93 mg/g, the content of the phlorizin is greater than 32.76 mg/g, the antioxidant value of the trilobatin is greater than 53.29%, and the antioxidant value of the phlorizin is greater than 42.90%. According to the extraction method disclosed by the invention, the liquid-to-solid ratio and the ethanol concentration are effectively reduced, the extraction time is shortened, the use amount of an organic solvent is reduced, the extraction efficiency is greatly improved, and besides, the antioxidant values of the trilobatin and the phlorizin are remarkably increased.

Description

technical field [0001] The invention relates to a method for extracting trilobatin and phlorizin from plants, in particular to a method for extracting trilobatin and phloridin from leaves of Coedeum japonicus and belongs to the field of extraction of trilobatin and phloridin. Background technique [0002] Lithocarpus litseifolius (Hance) Chun, also known as sweet tea, is a natural plant rich in active ingredients. Among them, flavonoids are one of the main chemical components of sweet tea, mainly including phlorizin and trilobatin, which belong to dihydrochalcones in sweet tea flavonoids, and have high development value. According to reports, trilobatin and phlorizin have the functions of lowering blood sugar, anti-oxidation, anti-cancer, anti-aging, etc., and are safe and non-toxic, and have great application value for health functions. [0003] So far, there are few research reports on the extraction methods of sweet tea trilobatin and phlorizin. Among them, the Chinese ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 甘人友刘毅刘宏艳夏宇郭欢李航
Owner CHENGDU AGRI SCI & TECH CENT
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