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Use of tipranavir in the preparation of cancer therapeutic drugs that kill tumor stem cells and tumor cells

A tumor stem cell and cancer treatment technology, which is applied in the field of preparation of cancer therapeutic drugs that kill tumor stem cells and tumor cells, can solve the problems of no anti-tumor application and no literature reports on application, and achieve the effect of improving the quality of life and improving the effect.

Active Publication Date: 2022-02-11
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the application of Tipranavir in the preparation of drugs for the treatment of tumor stem cells and tumor cells has not been reported in the literature, and has not been used in anti-tumor

Method used

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  • Use of tipranavir in the preparation of cancer therapeutic drugs that kill tumor stem cells and tumor cells
  • Use of tipranavir in the preparation of cancer therapeutic drugs that kill tumor stem cells and tumor cells
  • Use of tipranavir in the preparation of cancer therapeutic drugs that kill tumor stem cells and tumor cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Tipranavir can effectively kill human gastric cancer stem cells at a low concentration, and it is time- and concentration-dependent.

[0052] Two lines of gastric cancer stem cells (denoted as GCSC1 and GCSC2) derived from different gastric cancer patients were taken, and the cell concentrations were both (1×10 5 cells / mL, 100μl), inoculated in 96-well plate, three replicate wells in each group, treated GCSC1 with different concentrations of Tipranavir (0uM, 0.5uM, 1.0uM, 2.5uM, 5.0uM, 10uM, 20uM, 40uM, 100uM) And GCSC2 cells 24hr, 48hr, 72hr, DMSO as a control. CCK-8 was added to measure the absorbance value (A450) at a wavelength of 450 nm, and the cell growth curves of each group were drawn. Detect the killing effect of Tipranavir on gastric cancer stem cells (GCSC1 and GCSC2), and calculate its IC 50 , see the result figure 1 ( figure 1 , cell viability cell activity; concentration concentration).

[0053] The results showed that Tipranavir could eff...

Embodiment 2

[0054] Example 2: The effect of Tipranavir on killing gastric cancer stem cells is significantly better than that of the existing first-line drug combined with 5-FU and cisplatin.

[0055] Two lines of gastric cancer stem cells (denoted as GCSC1 and GCSC2) derived from different gastric cancer patients (1×10 5 cells / mL, 100 μl) were inoculated in 96-well plates, and each group had three replicate wells, and GCSC1 and GCSC2 cells were treated with Tipranavir (10uM) and 5-FU+cisplatin (5-FU: 2.5μM; cisplatin: 4μM) for 72hr, respectively, DMSO served as a control. CCK-8 was added to measure the absorbance value (A450) at a wavelength of 450 nm, and the cell growth curves of each group were drawn. Comparison of the killing effects of Tipranavir and 5-FU+cisplatin on gastric cancer stem cells (GCSC1 and GCSC2). see results figure 2 ( figure 2 , cell viability cell activity).

[0056] The results showed that the effect of Tipranavir on killing gastric cancer stem cells was si...

Embodiment 3

[0057] Example 3: Tipranavir can effectively kill gastric cancer cells, and its effect is also significantly better than the combination of 5-FU and cisplatin, and has no toxic side effects on normal gastric epithelial cells, while the combination of 5-FU and cisplatin has no effect on normal gastric epithelial cells. Cell toxicity.

[0058] Four gastric cancer cell lines (AGS, HGC-27, MGC-803, BGC-823) and normal gastric epithelial cell line (GES-1) (1×10 5 cells / mL, 100 μl) were inoculated in 96-well plates, and each group had three replicate wells, and the cells of each strain were treated with Tipranavir (10uM) and 5-FU+cisplatin (5-FU: 2.5μM; cisplatin: 4μM) for 72hr, DMSO as comparison. CCK-8 was added to measure the absorbance value (A450) at a wavelength of 450 nm, and the cell growth curves of each group were drawn. To compare the killing effects and side effects of Tipranavir and 5-FU+cisplatin on gastric cancer cells (AGS, HGC-27, MGC-803, BGC-823) and normal gast...

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Abstract

The invention discloses the use of Tipranavir in the preparation of cancer treatment drugs for killing tumor stem cells and tumor cells, and relates to the technical field of medicine. The invention also discloses a cancer treatment drug, which can kill tumor stem cells and tumor cells; the drug uses Tipranavir as the main active ingredient and contains a pharmaceutically acceptable carrier. The present invention provides for the first time that Tipranavir can be used to prepare an anticancer drug that kills tumor stem cells and tumor cells. Its anti-gastric cancer effect is significantly better than the existing first-line treatment drug for gastric cancer (combined use of 5-FU and cisplatin), and has no obvious side effects. The toxic and side effects are significantly less than the first-line treatment of gastric cancer (5-FU and cisplatin combined), which will provide new drugs and solutions for tumor treatment targeting tumor stem cells and cure tumors, and have a positive effect on improving the effect of tumor treatment and the quality of life of patients. important meaning.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of Tipranavir in the preparation of cancer therapeutic drugs for killing tumor stem cells and tumor cells. Background technique [0002] Malignant tumors are diseases with high morbidity and high mortality that seriously endanger human health. For example, gastric cancer (GC) is the most common malignant tumor of the digestive system, its incidence rate ranks fifth among all malignant tumors in the world, and its mortality rate ranks third among the causes of malignant tumors. my country is a high-incidence area of ​​gastric cancer, with high morbidity and mortality, both ranking second in malignant tumors. The principle of gastric cancer treatment is comprehensive treatment based on surgical treatment. In my country, the early diagnosis rate of gastric cancer is still relatively low. Most patients are diagnosed with advanced tumors and lose the chance of surgi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4433A61K31/337A61P35/00
CPCA61K31/4433A61K31/337A61P35/00A61K2300/00
Inventor 付利熊继先
Owner SHENZHEN UNIV
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