Device and method for detecting concentration of trace superoxide ion free radicals in water by adopting chemiluminescence reagent

Abstract

The invention relates to a device and a method for detecting the concentration of trace superoxide ion free radicals in water by adopting a chemiluminescence reagent. The device comprises a to-be-detected water body container bottle, a chemiluminescence reagent container bottle, a peristaltic pump, a spiral luminescent tube, a photon counter, a computer and a waste liquid bottle. And the to-be-detected water body container bottle and the chemiluminescence reagent container bottle are respectively communicated with the liquid inlet end of the spiral luminescent tube through the peristaltic pump. The photon counter is arranged on one side of the spiral light-emitting tube and used for recording light signals in the spiral light-emitting tube. The photon counter is connected with the computer. The waste liquid bottle is connected with the liquid outlet end of the spiral luminous tube. And a chemiluminescence reagent solution is contained in the chemiluminescence reagent container bottle.According to the method, a chemiluminescence reagent reacts with a to-be-detected water body solution containing superoxide ions to generate an optical signal, and primary attenuation fitting is carried out according to the dynamic track of the optical signal to deduce the concentration of the superoxide ions in the to-be-detected water body.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a device and method for detecting the concentration of trace superoxide ion free radicals in water by using a chemiluminescence reagent. Background technique [0002] In recent years, advanced oxidation technology has been widely used in the field of water treatment. This technology uses the generated active oxygen species to oxidize pollutants in water to achieve the purpose of purifying water. superoxide radical (O 2 ·- ) has become an important reactive oxygen species in the aquatic environment due to its reducing properties. Oxygen forms superoxide ions by accepting excited state electrons from electron donors. Superoxide ions can also combine with hydrogen ions to form hydroperoxy radicals (HO 2 · ) form, its acidity coefficient pKa is 4.48. Due to the existence of lone pairs of electrons, superoxide ions are very unstable in water and will decay through self-c...

AI Technical Summary

Problems solved by technology

Chemiluminescence has become the first choice for the detection of trace superoxide ions in environmental water due to its high sensitivity and simple operation. However, the existing luminescent reagents such as lucigenin and luminol have problems such as being easily interfered by metal ions and having poor selectivity.

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