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Single cell extraction method

An extraction method and cell technology, applied in chemical instruments and methods, biochemical equipment and methods, biochemical instruments, etc., can solve problems such as the destruction of living cells and many impurities in cell extracts, and achieve the effect of high extraction accuracy

Pending Publication Date: 2021-01-29
MEDCAPTAIN MEDICAL TECH
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Problems solved by technology

The cell extract obtained by this method not only has many impurities, but also destroys the living cells in the process of baking the cell membrane. Therefore, it is necessary to develop an extraction method that can accurately extract labeled cells to improve the quality of the cell extract. the purity of

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Embodiment Construction

[0030] Below in conjunction with embodiment and attached Figures 1 to 5 The present invention is further described.

[0031] The cell extraction system involved in the present invention includes a hardware device digital PCR cell separation chip (hereinafter referred to as "cell separation chip" or "chip") and a digital PCR cell separation tip (hereinafter referred to as "cell separation tip" or "tip" ) and a control system, the control system is used to control the positioning of the gun head to the target cell, and extract the target cell through the gun head. The specific method for extracting a single cell is: first drop the cell solution with labeled cells onto the cell separation chip, and the cell solution is automatically tiled on the cell separation chip to form a solution film; then locate the target cell in the fluorescence system, and finally Under the control of the control system, the cell separation tip runs to a specific position to absorb target cells. In t...

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Abstract

The invention discloses a single cell extraction method which specifically comprises the following steps: dripping a cell solution with labeled cells onto a cell separation chip, and automatically paving the cell solution on the cell separation chip to form a solution film; then positioning target cells in a fluorescence system, and finally, under the control of the control system, moving a cell separation gun head to a specific position to suck out the target cells. Through the extraction method provided by the invention, impurities in the cell extracting solution can be reduced, and the cellextracting solution with higher purity can be obtained, so that subsequent detection and analysis are facilitated.

Description

technical field [0001] The invention relates to a biomedical detection, in particular to a single cell extraction method. Background technique [0002] At present, most of the extraction of labeled tissue cells is by laser cutting method, that is, the tissue cells are first coated on a glass slide, then the cell membrane layer is dried, and finally the required calibration target is cut out by laser. The cell extract obtained by this method not only has many impurities, but also destroys the living cells in the process of baking the cell membrane. Therefore, it is necessary to develop an extraction method that can accurately extract labeled cells to improve the quality of the cell extract. purity. Contents of the invention [0003] In order to solve the problems existing in the prior art, the present invention provides a method for extracting a single cell, thereby reducing the impurities in the cell extract and facilitating subsequent detection and analysis. [0004] In...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/00B01L3/02
CPCC12M47/04B01L3/0213
Inventor 刘杰吕才树王海吴林涛
Owner MEDCAPTAIN MEDICAL TECH
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