Rhodotorula mucilaginosa SYM-1 as well as screening method and application thereof
A technology of SYM-1 and yeast, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of secondary pollution, water anoxic, non-degradable, etc., and achieve high degradation ability , Strong antibacterial effect, good development and application potential effect
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Embodiment 1
[0033] Embodiment 1, Rhodotorula colloides ( Rhodotorula mucilaginosa ) R-1 strain experiment:
[0034] 1 Materials and reagents
[0035] 1.1 Test material
[0036] Seawater and sea mud samples were taken from Lianyungang waters such as Rudong, Qidong, Cheniushan Island, and the estuary of the Guanhe River.
[0037] 1.2 Medium
[0038] Yeast enrichment medium: YEPD liquid medium.
[0039] Yeast isolation medium: YEPD medium: glucose (Sucrose) 20 g, yeast powder (Yeast extract) 3 g, tryptone 10 g, agar powder 20 g, water 1000ml, adjust pH 7.0.
[0040] Yeast purification medium: PDA medium.
[0041] Vibrio activation medium: 2216E medium.
[0042] Ammonia nitrogen degradation medium: glucose 10g, (NH 4 ) 2 SO 4 235.8g, NaCl 1g, MgSO 4 ˙7H 2 O 1g, K 2 HPO 4 1g, FeSO 4 ˙7H 2 O 0.4g, trace element solution 1mL, water 1000mL, pH 7.2-7.4. (Ammonia nitrogen concentration: 50 mg / L)
[0043] Nitrite degradation medium: glucose 10g, NaNO 2 0.075g, NaCl 1g, MgSO 4 ...
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