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Rhodotorula mucilaginosa SYM-1 as well as screening method and application thereof

A technology of SYM-1 and yeast, applied in chemical instruments and methods, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of secondary pollution, water anoxic, non-degradable, etc., and achieve high degradation ability , Strong antibacterial effect, good development and application potential effect

Active Publication Date: 2021-02-05
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the prevalence of high-density, high-feeding intensive farming methods, organic matter, animal manure, and residual bait in the water continue to accumulate, causing the ammonia nitrogen content to gradually exceed the water body's capacity. In addition, through sewage discharge, surface runoff, atmospheric deposition, The exogenous ammonia nitrogen entering the water body through pesticide residues and other methods is also increasing. The nitrite and ammonia nitrogen content in the water body are too high and cannot be degraded, which will cause problems such as water hypoxia. cause the animal to choke and die
At present, the pollution of nitrite and ammonia nitrogen in aquaculture water is mainly controlled by physical, chemical, and biological methods. The physical method has complicated steps and high cost, and is only suitable for the degradation of low-concentration nitrite and ammonia nitrogen (Zhang Chao et al. , 2020); the chemical method to degrade nitrite and ammonia nitrogen requires the help of related chemical substances, which requires a long reaction time and is prone to secondary pollution and other problems (Fu Jishuai et al., 2020); and the use of microorganisms to restore aquaculture water The method has been paid more and more attention because of its non-toxic side effects, short action time, and no pollution (Shi Dalin et al., 2009)

Method used

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  • Rhodotorula mucilaginosa SYM-1 as well as screening method and application thereof
  • Rhodotorula mucilaginosa SYM-1 as well as screening method and application thereof
  • Rhodotorula mucilaginosa SYM-1 as well as screening method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, Rhodotorula colloides ( Rhodotorula mucilaginosa ) R-1 strain experiment:

[0034] 1 Materials and reagents

[0035] 1.1 Test material

[0036] Seawater and sea mud samples were taken from Lianyungang waters such as Rudong, Qidong, Cheniushan Island, and the estuary of the Guanhe River.

[0037] 1.2 Medium

[0038] Yeast enrichment medium: YEPD liquid medium.

[0039] Yeast isolation medium: YEPD medium: glucose (Sucrose) 20 g, yeast powder (Yeast extract) 3 g, tryptone 10 g, agar powder 20 g, water 1000ml, adjust pH 7.0.

[0040] Yeast purification medium: PDA medium.

[0041] Vibrio activation medium: 2216E medium.

[0042] Ammonia nitrogen degradation medium: glucose 10g, (NH 4 ) 2 SO 4 235.8g, NaCl 1g, MgSO 4 ˙7H 2 O 1g, K 2 HPO 4 1g, FeSO 4 ˙7H 2 O 0.4g, trace element solution 1mL, water 1000mL, pH 7.2-7.4. (Ammonia nitrogen concentration: 50 mg / L)

[0043] Nitrite degradation medium: glucose 10g, NaNO 2 0.075g, NaCl 1g, MgSO 4 ...

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Abstract

The invention discloses a rhodotorula mucilaginosa SYM-1, and the preservation number of the rhodotorula mucilaginosa SYM-1 is CCTCC NO: M 2020756. The invention further discloses a screening method and application of the rhodotorula mucilaginosa SYM-1. The rhodotorula mucilaginosa SYM-1 strain can be used for biological prevention and treatment of aquaculture diseases or aquaculture water pollution treatment, has a strong bacteriostatic effect on vibrio vulnificus, vibrio parahaemolyticus and vibrio anguillarum, has a good degradation effect on nitrite and ammonia nitrogen, and has a degradation rate of about 65% when the nitrite nitrogen concentration is 50 mg / L. When the ammonia nitrogen concentration is 50 mg / L, the degradation rate reaches about 97%, and the rhodotorula mucilaginosa has good development and application potential.

Description

technical field [0001] The present invention relates to a kind of new strain, especially a kind of Rhodotorula colloides ( Rhodotorula mucilaginosa ) SYM-1; the present invention also relates to the screening method and application of the strain SYM-1. Background technique [0002] Vibrio is one of the main pathogens causing bacterial diseases in aquaculture. The diseases caused by them have a wide range of prevalence and high incidence, and have caused huge economic damage to the aquaculture industry. With the rapid development of economic globalization and my country's foreign trade, the frequent trade and circulation of aquatic products has led to the acceleration of the spread of aquaculture diseases, and the infection of pathogenic Vibrio diseases has become the main cause of death of farmed fish, shrimp and shellfish. . The prevention and treatment of bacterial diseases in aquaculture currently mainly relies on antibiotics. Although antibiotics have the advantages of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12Q1/04C02F3/34A61K36/064A61P31/04C12R1/645C02F101/16C02F103/20
CPCC12Q1/04C02F3/347A61K36/064A61P31/04C02F2101/16C02F2101/166C02F2103/20C12R2001/645C12N1/145Y02A40/81
Inventor 马桂珍张瑶李文进许建和李霁虹彭云汤曼利
Owner JIANGSU OCEAN UNIV
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