Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lung adenocarcinoma related diagnostic marker and application thereof

A diagnostic marker and lung cancer technology, applied in the field of diagnostic markers, can solve problems such as insufficient complete reversal of immunosuppression

Active Publication Date: 2021-02-05
JIANGSU CANCER HOSPITAL
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For reversing the immune escape of tumors, immune checkpoint inhibitors such as PD-1, PD-L1, and CTLA-4 perform well, but these targets are far from enough to completely reverse the immunosuppressive state of the tumor immune microenvironment, and related drugs can be used alone For patients with advanced lung adenocarcinoma, the effective rate is only 20%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lung adenocarcinoma related diagnostic marker and application thereof
  • Lung adenocarcinoma related diagnostic marker and application thereof
  • Lung adenocarcinoma related diagnostic marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]219 cases of lung adenocarcinoma tissue section specimens and 32 corresponding paracancerous tissue disease section specimens, all of which were obtained from patients hospitalized for surgery during 2007-2017 in the Department of Pathology, Cancer Hospital Affiliated to Nanjing Medical University. All cases were confirmed by pathology and histology by two pathologists. The patients had not received immunotherapy, chemotherapy or radiotherapy before operation, and the clinical case information was detailed and complete.

[0040] All tissue samples were routinely fixed in 10% neutral formalin, embedded in paraffin, and the wax blocks were screened without obvious defects. The pathology department was entrusted to make tissue chips with a thickness of 4 mm, and they were stored in a refrigerator at 4°C for later use.

[0041] 1.1 Fabrication of tissue chips:

[0042] (1) According to the microscopic examination results of the HE-stained section, mark the representative can...

Embodiment 2

[0057] 2.1 siRNA design

[0058] Four different siRNA sequences of human IL1F5 and negative control siRNA (negative control, NC) were selected and designed and completed by bioinshine company. The sequences are as follows:

[0059]

[0060] 2.2 Cell Recovery:

[0061] (1) Take out the cryopreservation tube of H3255 cells from the -80°C refrigerator, put it in a 37°C water bath and shake it quickly, so that the cells are completely thawed within 1 min. Take out the cryotube for alcohol disinfection and put it into the ultra-clean bench. (2) Pipette the cell suspension into a 15mL centrifuge tube, add 10mL RPMI-1640 to AGS, SNU719, MKN1, H3255, MKN27 cells, mix well, and centrifuge at 1200rpm for 3min. (3) Pour off the supernatant, take 2 mL of culture medium containing 10% fetal bovine serum to resuspend the cells, transfer to a cell culture bottle, and place at 37°C, 5% CO 2 Cultured in a cell culture incubator, the culture medium was replaced once the next day, and the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a lung adenocarcinoma related diagnostic marker and an application thereof. The diagnostic marker is used for encoding IL1F5 protein and encoding mRNA of the IL1F5 protein. PCRdetection and tissue chip immunohistochemistry of a clinical lung adenocarcinoma sample prove that the expression of IL1F5 in the lung adenocarcinoma is increased, and survival analysis shows that the prognosis of a patient with higher expression of the IL1F5 is poorer. Influence of down-regulation of the IL1F5 gene expression by siRNA on biological behaviors such as lung adenocarcinoma cell proliferation and invasion is studied through in-vitro cell experiments, and it is found that a specific siRNA sequence can effectively inhibit expression of the IL1F5 protein in a human lung adenocarcinoma cell strain H1299. Under the condition of co-cultivation with CD8+T cells, the proliferation and invasion capacities of H1299 cells with reduced IL1F5 expression are reduced (no difference exists when the tumor cells are cultured alone).

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a diagnostic marker related to lung cancer and its application. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. In recent years, the pathological types of lung cancer in my country have quietly changed. The incidence of lung adenocarcinoma (LUAD) has risen to 70% of the total number of lung cancers, exceeding the proportion of lung adenosquamous carcinoma. leading position. With the advancement of surgery, chemotherapy, and radiotherapy, the survival rate of lung adenocarcinoma patients has been greatly improved in recent years. However, since lung adenocarcinoma is mostly peripheral, there are often no obvious clinical symptoms in the early stage, and it is often too late when the diagnosis is made, and the best period for surgery is missed. Moreover, the sensitivity of traditional chemotherapy and radiotherapy is limited, and duri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6886C12N15/11G01N33/574A61K31/713A61P35/00
CPCA61K31/713A61P35/00C12Q1/6886C12Q2600/118C12Q2600/158G01N33/57423G01N33/57484G01N2800/52
Inventor 陈辰黄兴于韶荣陆建伟戴立玲
Owner JIANGSU CANCER HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com