Rapid nondestructive detection method for plant root cancer

Abstract

The invention relates to a rapid nondestructive detection method for plant root cancer. The detection method comprises the following steps of providing agrobacterium DNA obtained from a plant to be detected, wherein the concentration of the agrobacterium DNA is 20 ng / [mu]l; carrying out PCR amplification by taking the agrobacterium DNA as a template, wherein the amplification cycle numbers are 20-25 times, 25-30 times and 30-40 times respectively; performing agarose gel electrophoresis on an amplification product, and obtaining an amplification result through a gel imaging system; and obtaining the attack degree of the to-be-detected plant root cancer according to the amplification result. The method disclosed by the invention can provide a qualitative judgment basis for detection and grading of host plants infected by agrobacterium, and meanwhile, can provide an accurate evaluation means for targeted prevention and treatment according to different infection severities.

Description

technical field [0001] The invention relates to a rapid non-destructive detection method for plant root cancer. Background technique [0002] Root cancer, also known as root nodule, is a soil-borne disease caused by Agrobacterium soil. It can cause disease in grapes, apples, pears, peaches, apricots and other fruit trees. Due to the many tumors, it is difficult to transport nutrients from the roots upwards. The tree body weakens, and the whole plant dies in severe cases. It mainly harms the root neck, main root, and lateral roots. After the infection, the leaves of the fruit trees are narrow and yellow, the tree vigor is weak, the growth is retarded, the life span is shortened, the fruit is stunted, the yield is reduced, and even death. Once this disease occurs, it is difficult to control and the harm is serious. However, due to the specific infection mode of Agrobacterium tumefaciens, the traditional agricultural, physical and chemical control methods have no good effect,...

AI Technical Summary

Problems solved by technology

At present, field detection can judge the degree of disease susceptibility through the shape of stems and roots, but there are misjudgments, and the identification of the incubation period cannot be carried out. In addition, since root nodules mainly reproduce in the roots, the roots need to be dug out for diagnosis, which will cause great damage to fruit trees.

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