Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus mucilaginosus and application thereof in agricultural production

A technology of Bacillus colloidus and Bacillus, which is applied in the screening and application of functional microorganisms, can solve the problems of lack of biological control research, and achieve the effects of agricultural health, improvement of soil environment, and environmental friendliness

Active Publication Date: 2021-02-09
QINGDAO LILIHUI BIOTECH
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Bacillus colioids has been discovered for more than 100 years. People have done in-depth research on the application of this bacteria in microbial fertilizers, mineral decomposition and bioflocculation, and have made certain achievements. However, in terms of biological control research is still lacking

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus mucilaginosus and application thereof in agricultural production
  • Bacillus mucilaginosus and application thereof in agricultural production
  • Bacillus mucilaginosus and application thereof in agricultural production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Isolation and screening of phosphorus-solubilizing microorganisms in the soil of embodiment 1

[0041] 1. Soil sample: Grape rhizosphere soil in Dazeshan District, Pingdu, Qingdao City, Shandong Province.

[0042] 2. Preparation of soil dilution:

[0043] Remove surface plant residues and surface soil 5cm-10cm, collect 10g samples from soil at each sampling point by multi-point collection method, and put them into sample bags. After drying in the shade, divide the soil sample into about 50g by quartering method; then place it in a 500ml Erlenmeyer flask containing 250ml sterilized PB buffer solution; shake at 30°C and 180rpm for 30min, let it stand for precipitation, and take the supernatant; Take 100ml of the supernatant, centrifuge at 12000rpm for 10min, and take the precipitate; suspend the precipitate in 50ml of sterilized PB buffer and centrifuge again at 12000rpm for 10min; after repeating twice, suspend the precipitate in 10ml of sterile water to obtain a soil s...

Embodiment 2A11

[0054] The identification of embodiment 2A11 bacterial strain

[0055] 1. Molecular biological identification

[0056] Pick a single colony of the A11 strain on the plate and place it in the nutrient broth medium, culture it at 37°C for 24 hours, then take 500ul of the fermentation broth of the strain, and use the kit to extract the genome of the strain. Using the genome as a template, primer sequences were designed to amplify the 16s rDNA sequence by PCR.

[0057] 1) Primer sequence:

[0058] A11F: AGGGTTTGATCCTGGCTCC;

[0059] A11R: GGTGGATTCTTGTTACGACTT.

[0060] 2) Reaction system (50μL)

[0061] Table 2 16s rDNA PCR amplification system

[0062]

[0063] 3) The 1% agarose gel electrophoresis profile of the PCR amplification product is as follows: figure 1 As shown, the length of the amplified 16s rDNA fragment is about 1500 bp, which is in line with the conventional 16s rDNA sequence length.

[0064] 4) PCR product sequencing

[0065] The amplified PCR products...

Embodiment 3

[0072] Example 3 Determination of Potassium Decomposing Ability of Bacillus gliosa LLH08

[0073] 3.1 Determination method

[0074] Prepare potassium feldspar liquid medium, and divide into 250mL Erlenmeyer flasks according to 95mL. The suspension of Bacillus coliformis LLH08 was transferred into the shake flask with 5% inoculum amount, and three parallels were performed at the same time, and a control group without inoculation was set, and the culture was shaken at 30° C. for 72 hours.

[0075] 3.2 Treatment of fermentation broth

[0076] Take 20mL of fermentation broth from each shake flask, centrifuge at 4°C and 5000r / min for 15min; put all the centrifuged supernatant into a 50mL digestion tube; add 5mL of concentrated sulfuric acid and 2mL of hydrogen peroxide solution to digest Cook in the oven, and repeatedly add 20% hydrogen peroxide solution several times until the viscous substance is completely digested, then dilute to 50mL with distilled water. K was determined u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of functional microorganism screening and application, in particular to novel bacillus mucilaginosus and an application thereof in agricultural production.The preservation number of the bacillus mucilaginosus is CCTCC NO: M2020163. The bacillus mucilaginosus can effectively improve the soil fertility, promote crop growth and increase the yield, has a very good prevention and treatment effect on plant diseases such as apple ring rot, brown spot and cucumber root-knot nematode disease, and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of screening and application of functional microorganisms, in particular to a colloid bacillus and its application in agricultural production. Background technique [0002] Microbial fertilizer is a new type of fertilizer, also known as biological bacterial fertilizer, bacterial agent, and bacterial fertilizer. Included in microbial fertilizers are many microorganisms that play important roles. Microbial fertilizers have the advantages of reducing soil compaction, improving soil fertilizer retention capacity, enhancing the utilization rate of alkaline nitrogen and other substances in the soil, increasing crop yield and improving quality. Compared with inorganic fertilizers and organic fertilizers, microbial fertilizers are fundamentally different, so microbial fertilizers cannot completely replace existing fertilizers. [0003] As early as the 1950s, microbiologists from the former Soviet Union discovered ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/60C05G3/80A01N63/22A01P3/00A01P5/00C12R1/07
CPCC05F11/08C05G3/60C05G3/80A01N63/22C12R2001/07C12N1/205Y02A40/10
Inventor 代庆海孙春龙王丽宁李倩倩林俊荣王凌云
Owner QINGDAO LILIHUI BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com