Human respiratory virus targeted enrichment capture probe set and application thereof

A technology for targeted enrichment and capture of probes for detection, identification and assessment of respiratory viruses
CN112342270AInactive Publication Date: 2021-02-09INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
Publication Date
2021-02-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of human respiratory virus diagnosis, and provides a method for generating a targeted enrichment capture probe set for human respiratory viruses. The method comprises the following steps of acquiring a gene sequence of a virus infecting a human respiratory tract; designing a probe through a sliding window method, and performing exhaustion of all possible nucleicacid sequences; filtering the obtained nucleic acid sequences; filtering out sequences highly homologous with the human genome in a nucleic acid sequence library; and clustering the probe sequences. The invention also relates to application of the probe set obtained through the method to preparation of a next-generation sequencing library of human respiratory viruses, so that whole genome sequenceinformation is obtained, the genome variation is identified, potential epidemic propagation characteristics of viruses are evaluated, and early warning and prevention and control of respiratory diseases are guided.
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Description

technical field

[0001] The invention relates to a human respiratory virus targeted enrichment capture probe set and its use for detecting, identifying and evaluating respiratory viruses. Background technique

[0002] Human respiratory virus is an important pathogen that causes human infection, and it is also the main pathogen of new and sudden infectious diseases. Viruses are prone to mutations, which may lead to changes in virulence, drug resistance, or transmission capabilities, and thus cause major public health problems due to rapid spread among the population. Rapid and accurate detection and identification of pathogens, especially obtaining their genome sequence characteristics, is the key to discovering whether there are mutations and new viruses, and then evaluating the potential epidemic characteristics of the virus, which is of great significance for the early warning and prevention of infectious diseases.

[0003] The common respiratory viruses that cause human r...

Claims

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