IFA neutralizing antibody detection method of PRRSV

An antibody detection and cell technology, applied in the biological field, can solve the problems of inability to evaluate PRRSV-specific antibody response and development
CN112362880APending Publication Date: 2021-02-12XINXIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
XINXIANG UNIV
Publication Date
2021-02-12

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Abstract

The invention discloses an IFA neutralizing antibody detection method for PRRSV, and belongs to the technical field of molecular biological detection. The invention discloses an IFA neutralizing antibody detection method of PRRSV. The method comprises the following steps: inoculating an isopyknic PRRSV virus solution and clinical serum onto Marc145 cells, immobilizing, adding a primary antibody, adding a secondary antibody, and observing a result. According to the method, target cells are infected after interaction of whole viruses and antibodies, and the situation of interaction of the viruses and the neutralizing antibodies is truly reflected.
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Description

technical field

[0001] The invention relates to the field of biotechnology, and more specifically relates to a method for detecting a PRRSV IFA neutralizing antibody. Background technique

[0002] Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive-sense enveloped RNA virus. Virions consist of a nucleocapsid core constructed of a nucleocapsid protein (encoded by open reading frame 7 or ORF7) combined with viral RNA. The nucleocapsid is surrounded by a lipid envelope, which contains six structural proteins: glycoproteins GP2 (ORF2a), GP3 (ORF3), GP4 (ORF4) and GP5 (ORF5), and non-glycosylated proteins M (ORF6) and E(ORF2b). GP5 and M were found to be the most abundant proteins in the envelope, while other envelope proteins were present in lower amounts. Therefore, the antibody response after PRRSV infection is very complex, which can be generally divided into neutralizing antibody (NA) and non-neutralizing antibody (NNA). Some studies have shown that ...

Claims

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