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Hydrangea breeding method

A technology of hydrangea and cuttings, applied in the field of hydrangea breeding, can solve the problems of high cost, complicated procedures, and complicated operation, and achieve the effect of large number of roots, high rooting rate, and simple operation

Pending Publication Date: 2021-02-19
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cutting propagation requires a specific substrate, high hormone concentration and specific culture environment, which is costly and relatively complicated to operate. The use of excessively high concentrations of hormones in the test process will also cause environmental pollution.
The procedures involved in tissue culture are more complicated, and the experimental operation needs to be completed under laboratory conditions, and the cultivation process needs to be completed under sterile conditions. Different culture stages require different culture media, which is not of general promotion value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The branches of the same year with the same growth were selected as the research object. Cut each branch into upper, middle and lower cuttings, and keep two nodes for each cutting, with a length of 10 cm. When pruning cuttings, the incision is smooth, the upper incision is 2 cm from the upper end node, and the incision is flat; the lower end incision is 2 cm from the lower end node, the incision is an oblique incision, and the incision angle is 45 degrees. Keep 2 leaves at the node at the upper end of the cuttings, keep 1 / 2 the size of the leaves, and remove all the leaves at the node at the lower end.

[0048] Take 30 cuttings and culture them in sterile water, and replace the sterile water every 24 hours. The light and dark culture conditions are 16h / 8h, and the temperature is set to 25°C under light culture conditions, and 20°C under dark culture conditions.

Embodiment 2

[0050] The branches of the same year with the same growth were selected as the research object. Cut each branch into upper, middle and lower cuttings, and keep two nodes for each cutting, with a length of 10 cm. When pruning cuttings, the incision is smooth, the upper incision is 2 cm from the upper end node, and the incision is flat; the lower end incision is 2 cm from the lower end node, the incision is an oblique incision, and the incision angle is 45 degrees. Keep 2 leaves at the node at the upper end of the cuttings, keep 1 / 2 the size of the leaves, and remove all the leaves at the node at the lower end.

[0051] Take 30 cuttings for culture in sterile water, replace the sterile water every 24 hours, the concentration of NAA in the sterile water is 5mg / L, the light and dark culture conditions are 16h / 8h, and the temperature is set to 25°C under light culture conditions, The temperature was set at 20 °C under dark culture conditions.

reference example 1

[0053] The branches of the same year with the same growth were selected as the research object. Cut each branch into upper, middle and lower cuttings, and keep two nodes for each cutting, with a length of 10 cm. When pruning cuttings, the incision is smooth, the upper incision is 2 cm from the upper end node, and the incision is flat; the lower end incision is 2 cm from the lower end node, the incision is an oblique incision, and the incision angle is 45 degrees. Keep 2 leaves at the node at the upper end of the cuttings, keep 1 / 2 the size of the leaves, and remove all the leaves at the node at the lower end.

[0054] Take 30 cuttings for culture in sterile water, replace the sterile water every 24 hours, the concentration of NAA in the sterile water is 10mg / L, the light and dark culture conditions are 16h / 8h, and the temperature is set to 25°C under the light culture conditions, The temperature was set at 20 °C under dark culture conditions.

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Abstract

The invention provides a hydrangea breeding method. The hydrangea breeding method comprises the following steps of cultivating hydrangea cuttings with at least two reserved sections in a water environment to obtain hydroponic seedlings with adventitious roots, and inserting the sections at the lower ends of the cuttings into water. Compared with a hydrangea cutting and tissue culture breeding mode, the hydrangea breeding method is easy to operate, does not need strict breeding conditions, and is low in cost, free of pollution, high in hydrangea rooting rate and large in rooting number.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a hydrangea breeding method. Background technique [0002] Hydrangea macrophylla (Hydrangea macrophylla) is a small deciduous shrub of the genus Hydrangea in the family Hydrangea, also known as Hydrangea, Hydrangea, etc. Because of its extensive management, fewer pests and diseases, and large inflorescences, it has been widely used in urban beautification, garden landscaping, potted plants and cut flowers. The hydrangea industry has developed rapidly in China, and hydrangea has also become popular among people. One of the flower varieties. [0003] At present, most of the domestically grown hydrangeas are imported from abroad. The most ornamental part of hydrangea is the huge inflorescence composed of many sterile flowers. Therefore, the higher the ornamental value, the more difficult it is to obtain its seeds. At the same time, hydrangea seeds are extremely small in sh...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G2/10
CPCA01G31/00A01G2/10A01G22/60
Inventor 彭继庆薛超董旭杰曹福祥曹基武吴毅
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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