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Heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as preparation method and application of heat-resistant protective agent

A technology of heat-resistant protective agent and canine adenovirus, which is applied in antiviral agents, biochemical equipment and methods, viruses, etc., can solve the problems of temperature sensitivity of virus antigens, poor heat-resistant protection effect, and high requirements for storage conditions, and achieve Good economic and social benefits, good heat resistance and protection effects, and broad application prospects

Active Publication Date: 2021-02-19
JINYUBAOLING BIO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps prevent damage from losing important viruses like feline caliciviridae (FCAV) or porcinerus rotavis due to freezing drying processes performed on different types of veterinary medications such as horse sickness treatments. By mixing certain ingredients with this new method, it provides an effective way to make sure these drugs are safe while still maintain their ability to stimulate immune responses against specific diseases.

Problems solved by technology

This patents discuss different types of animal reservoir fluids like blood, urine, sputum, swabs, sporides, bitigens, and others. These liquids contain harmful microorganisms responsible for veterinary concern worldwide including avid herds, rodworms, horseshoe warts, tickles, dementias, and prurence syndrome. Current methods involve administering therapeutoxvirus based therapies against both epidemics and nonfatal cases associated with exposure to specific strains found around human populations during timespan distribution.

Method used

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  • Heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as preparation method and application of heat-resistant protective agent
  • Heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as preparation method and application of heat-resistant protective agent
  • Heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as preparation method and application of heat-resistant protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine heat-resistant protective agent

[0042] This embodiment aims to prepare a heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus, and canine parainfluenza quadruple live vaccine. After drying, the protection is implemented in the storage stage to ensure the immune effect of the vaccine. The preparation method of the heat-resistant protective agent specifically includes the following steps:

[0043] 1.1. Preparation of the first solution: according to the composition content of the heat-resistant protective agent (heat-resistant protective agent 1, 2, 3, 4, 5) shown in the following table 1 (wherein % represents the mass volume percentage content, that is, g / 100ml, The same below), accurately weigh the required amount of gelatin in the quantitative water for injection, heat it in a 60°C water bath and keep s...

Embodiment 2

[0049] Embodiment 2: preparation canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine

[0050] This embodiment utilizes the protective agent 1, protective agent 2, protective agent 3, protective agent 4 and protective agent 5 prepared in Example 1 above as heat-resistant protective agents of live vaccines to prepare canine distemper, canine parvovirus, and canine adenovirus , canine parainfluenza quadruple live vaccine, the compound method of quadruple live vaccine comprises the following steps:

[0051] 2.1. Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza virus venom

[0052] 2.1.1. Preparation of canine distemper virus venom

[0053] Propagation of canine distemper virus virus liquid: Take a single layer of well-grown Vero cells (provided by the National Engineering Laboratory of Jinyu Baoling Biopharmaceutical Co., Ltd.), discard the growth liquid, digest the cells, and dilute the ce...

Embodiment 3

[0096] Embodiment 3: Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine heat-resistant protective agent

[0097]In this embodiment, according to the same method as steps 1.1-1.3 in Example 1, according to the composition and content of the heat-resistant protective agent listed in Table 12 below, the quadruple live vaccine resistant to canine distemper, canine parvovirus, canine adenovirus, and canine parainfluenza was prepared. The thermal protectants are named protectant 6, protectant 7 and protectant 8 respectively. Utilize protective agent 6, protective agent 7 and protective agent 8 to prepare canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine respectively according to the method identical with step 2.3-2.4 in embodiment 2, wherein antigen: Protectant = 3:1. Subsequently, according to the same method as in Example 2, each virus drop before and after freeze-dry...

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PUM

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Abstract

The invention discloses a heat-resistant protective agent for a canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as a preparation method and application of the heat-resistant protective agent and belongs to the technical field of heat-resistant protective agents for live vaccines in the field of biological medicines. The heat-resistantprotective agent comprises 7%-10% of cane sugar, 3%-4% of gelatin, 8%-10.0% of trehalose, 6%-8% of peptone, 2%-4% of glycine and 4%-6% of PEG, the components are simple, the raw materials are easy toobtain, the preparation method is simple, and the heat-resistant protective agent can effectively reduce the loss of various virus antigens in the freeze-drying process when used for preparing thecanine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine, and can ensure the immune efficacy and long-term stable storage of the canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine.

Description

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Claims

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Application Information

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Owner JINYUBAOLING BIO PHARMA CO LTD
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