Heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus and canine parainfluenza quadruple live vaccine as well as preparation method and application of heat-resistant protective agent
A technology of heat-resistant protective agent and canine adenovirus, which is applied in antiviral agents, biochemical equipment and methods, viruses, etc., can solve the problems of temperature sensitivity of virus antigens, poor heat-resistant protection effect, and high requirements for storage conditions, and achieve Good economic and social benefits, good heat resistance and protection effects, and broad application prospects
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Embodiment 1
[0041] Embodiment 1: Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine heat-resistant protective agent
[0042] This embodiment aims to prepare a heat-resistant protective agent for canine distemper, canine parvovirus, canine adenovirus, and canine parainfluenza quadruple live vaccine. After drying, the protection is implemented in the storage stage to ensure the immune effect of the vaccine. The preparation method of the heat-resistant protective agent specifically includes the following steps:
[0043] 1.1. Preparation of the first solution: according to the composition content of the heat-resistant protective agent (heat-resistant protective agent 1, 2, 3, 4, 5) shown in the following table 1 (wherein % represents the mass volume percentage content, that is, g / 100ml, The same below), accurately weigh the required amount of gelatin in the quantitative water for injection, heat it in a 60°C water bath and keep s...
Embodiment 2
[0049] Embodiment 2: preparation canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine
[0050] This embodiment utilizes the protective agent 1, protective agent 2, protective agent 3, protective agent 4 and protective agent 5 prepared in Example 1 above as heat-resistant protective agents of live vaccines to prepare canine distemper, canine parvovirus, and canine adenovirus , canine parainfluenza quadruple live vaccine, the compound method of quadruple live vaccine comprises the following steps:
[0051] 2.1. Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza virus venom
[0052] 2.1.1. Preparation of canine distemper virus venom
[0053] Propagation of canine distemper virus virus liquid: Take a single layer of well-grown Vero cells (provided by the National Engineering Laboratory of Jinyu Baoling Biopharmaceutical Co., Ltd.), discard the growth liquid, digest the cells, and dilute the ce...
Embodiment 3
[0096] Embodiment 3: Preparation of canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine heat-resistant protective agent
[0097]In this embodiment, according to the same method as steps 1.1-1.3 in Example 1, according to the composition and content of the heat-resistant protective agent listed in Table 12 below, the quadruple live vaccine resistant to canine distemper, canine parvovirus, canine adenovirus, and canine parainfluenza was prepared. The thermal protectants are named protectant 6, protectant 7 and protectant 8 respectively. Utilize protective agent 6, protective agent 7 and protective agent 8 to prepare canine distemper, canine parvovirus, canine adenovirus, canine parainfluenza quadruple live vaccine respectively according to the method identical with step 2.3-2.4 in embodiment 2, wherein antigen: Protectant = 3:1. Subsequently, according to the same method as in Example 2, each virus drop before and after freeze-dry...
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