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Ventricular catheterization for constructing rat model for researching PHN pinecone excision and construction method thereof

A rat model and construction method technology, applied in veterinary instruments, veterinary surgery, medical science, etc., can solve the problems affecting the construction of rat models, large rat surgery, inconvenient operation, etc., to improve experimental accuracy and The effect of rat survival rate, reduction of catheter volume and improvement of survival rate

Pending Publication Date: 2021-02-23
GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing pinealectomy rat model used to study PHN, in order to save costs, most of the catheters used in the ventricle are self-made or purchased from Reward Company. The volume of such catheters is too large for rat surgery. Inconvenient to operate, therefore, the survival rate of rats after surgery is not high, which seriously affects the construction of rat models

Method used

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  • Ventricular catheterization for constructing rat model for researching PHN pinecone excision and construction method thereof
  • Ventricular catheterization for constructing rat model for researching PHN pinecone excision and construction method thereof
  • Ventricular catheterization for constructing rat model for researching PHN pinecone excision and construction method thereof

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Experimental program
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Effect test

Embodiment 2

[0064] Embodiment two is based on the structure of the ventricle catheter 1 in embodiment one to construct an animal model, and the specific content is as follows:

[0065] 1. Experimental animals

[0066] 88 healthy adult (3-month-old) male or female Wistar rats, each weighing about 200 g, were raised in an environment of 22° C., and provided with feed and drinking water normally. Rats were illuminated with a 40-watt daylight-colored fluorescent lamp, placed in a specially-designed, well-ventilated place, with a light-to-dark cycle ratio of 12h:12h, providing about 400 lux of energy. All the above operating procedures were approved by the Experimental Animal Protection and Use Committee of Guiyang Medical College.

[0067] 2. Build an animal model

[0068] S1, the prepared Wistar rats are randomly divided into non-treatment group (40) and treatment group (48);

[0069] S11, the non-treatment group rats in the step S1 are divided into four groups on average: A experimental ...

Embodiment 3

[0090] The third embodiment is described on the basis of the structure of the intraventricular catheter 1 in the first embodiment, and aims to clarify the method for measuring the mechanical pain threshold of rats.

[0091] The rats were placed on the mesh suspension board respectively, and after 20 minutes of acclimatization, the test probe was vertically applied 0.6g or 4.0g of mechanical stimulation to the hind feet for 5s. Rats were ranked according to their responses as follows: 0, no response; 1, away from the von Frey probe; 2, flinching or licking the hind paw. Each hind paw was stimulated with the same intensity after an interval of 3 minutes, and a total of 6 stimulations were given, and the average value was used as the pain-related score. In the experiment, when the Von Frey probe stimulated the rats, since it was difficult to distinguish the pain-related responses from the walking behavior, a pain-related score of 0.5 or higher could be regarded as hyperalgesia. ...

experiment example 4

[0093] Experimental Example 4 is described on the basis of the rat model constructed in Example 2, and aims to clarify the method for measuring the thermal paw withdrawal latency of rats.

[0094] The heat paw withdrawal latency of rats is measured by using an infrared heat radiation heat pain tester. The specific process is as follows: After the rat is placed in the experimental environment for 20 minutes, the hind paw is exposed to the light beam of the heat radiation heat pain tester. The average value of the measurements was used to determine the baseline latency, with a baseline of infrared intensity of radiant heat set to 1, about 20 seconds under normal conditions.

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Abstract

The invention relates to the technical field of animal model construction, and particularly relates to a ventricular catheterization for constructing a rat model for researching PHN pinecone excisionand a construction method thereof. The ventricular catheterization consists of an outer tube, an inner core and an inner needle, wherein one end of the outer tube is sleeved with a bonding head; one end, far away from the outer tube, of the bonding head is a hollow connecting rod; one end, far away from the outer tube, of the hollow connecting rod is provided with an axial sliding chute; and the tail end of the axial sliding chute is tangentially provided with a transverse sliding chute. According to the ventricular catheterization, the most critical ventricular catheterization structure in ananimal model is optimized, the catheterization stability is improved while the catheterization volume is reduced, and the operation difficulty is lowered; and compared with an existing catheterization, the ventricular catheterization designed by the invention has the advantages that the length of the outer tube can be regulated according to the body types of rats of different batches, so that theinsertion depth accuracy is guaranteed, and the experiment accuracy and the rat survival rate are improved. The rat model constructed by the invention can be used for effectively researching the influence of MT on the aspect of PHN pain, which indicates that the rat model constructed by the invention has a remarkable effect.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a ventricle catheter for researching the construction of a PHN pinealectomy rat model and a construction method thereof. Background technique [0002] Animal models refer to animal experimental objects and materials established in biomedical research that can simulate human diseases or abnormal functional states. [0003] HSV-1 and VZV are commonly used methods to induce hyperalgesia to reveal response variability in onset, duration, and severity of lesions. After inoculation with VZV, the magnitude of hyperalgesia was 30-40% of the baseline, and after inoculation with HSV-1, it was 80% of the baseline. There were also significant differences in related behaviors in animals inoculated with the two viruses. As the viral infection spreads to the central nervous system, HSV-1 was able to cause skin lesions and paralysis of the hind limbs, whereas no lesions or par...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 聂瑛洁张湘燕陈辉罗新华潘润桑
Owner GUIZHOU PROVINCIAL PEOPLES HOSPITAL
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