Biological detection method based on excitation light source

Abstract

The invention provides a biological detection method based on an excitation light source. The biological detection method comprises the following steps of: injecting a sample into a sample adding holeformed in a sample adding layer; connecting a gasket arranged below the sample adding layer with a pipeline layer at the lower end through a buckle structure, during use, pulling out the gasket alonga rail, pressing the sample adding layer downwards to connect the pipeline layer with the sample adding layer in a clamped mode, sequentially mixing a row of pricking needles arranged on the pipelinelayer with a reagent of the sample adding layer, and carrying out extraction and purification reaction in the pipeline layer, then introducing nucleic acid substances in the sample into an amplification bin arranged on the pipeline layer; enabling an excitation light module to emit exciting light with a preset wavelength to an amplification bin, carrying out reaction, then acquiring fluorescenceinformation through a fluorescence sensor to complete detection, and controlling the temperature in the amplification bin to be within a preset range by the temperature control module, thus enabling areagent of a chip device to reach an optimal reaction state.

Description

technical field [0001] The invention relates to the technical field of nucleic acid amplification instrument detection, in particular to a biological detection method based on an excitation light source. Background technique [0002] In recent years, fluorescence analysis technology has been widely used in fluorescence microscopy imaging and observation, and flow fluorescence detection and analysis. The required fluorescence spectrophotometer includes an excitation light device that inputs excitation energy, that is, an excitation light source. At present, fluorescence microscopy imaging systems generally use mercury lamps, xenon lamps and other high-energy, short-life full-band light sources, which are filtered by different filters to obtain excitation light sources of the corresponding required bands. However, the optical path system of this kind of excitation light source is complicated, the energy consumption of the light source is high, the efficiency loss is large, and...

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Problems solved by technology

[0004] In the prior art, some fluorescence detection achieves the purpose of multi-light path detection by adding a rotatable excitation light filter color wheel and an emission light color wheel in the optical path, but the setting of the above structure increases power consumption, and, in the fluorescence detection process In the process, due to the mechanical rotation, there are problems of misalignment or misalignment of light, and the instrument is large and clumsy, not easy to carry, and it will affect the progress of the experiment in the process of multiple corrections

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