Preparation method of dendrobium officinale extract

A technology of Dendrobium officinale and extract, which is applied in the field of preparation of Dendrobium officinale extract, can solve problems such as inability to meet cosmetic performance requirements, organic solvent residues, potential safety hazards, etc., achieve high antioxidant and moisturizing effects, simple operation, and high yield big effect

Inactive Publication Date: 2021-03-02
泉后(广州)生物科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used extraction methods include aqueous solution heating extraction, organic solvent extraction, etc., but the aqueous solution heating extraction needs to be carried out at high temperature, which is easy to destroy the active ingredients, and the organic solvent extraction solution has organic solvent residues, which has certain safety hazards
Chinese patent application CN111334540A discloses a method for extracting polysaccharides from Dendrobium officinale by biological fermentation. In this method, the polysaccharides from Dendrobium officinale are separated and purified by yeast fermentation, and the yield, protein clearance rate and decolorization rate are high. However, its moisturizing and anti-oxidation effects are average, and it still cannot meet the current consumer requirements for cosmetic performance.

Method used

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  • Preparation method of dendrobium officinale extract
  • Preparation method of dendrobium officinale extract
  • Preparation method of dendrobium officinale extract

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1 A kind of preparation method of dendrobium officinale extract

[0033] The preparation method of described Dendrobium officinale extract comprises the following steps:

[0034] S1. Beat the dried strips of Dendrobium candidum into small sections with a length less than 2cm, then put the small sections of Dendrobium candidum into an oven and dry them at 65°C for 3 hours, add them into an ultrafine pulverizer and crush them into powder, and sieve them through a 100-mesh sieve. Gained Dendrobium officinale powder is added in the fermentation tank (50L) that 30L water is housed by 10g / L, adds 0.5% sucrose again, sterilizes, obtains Dendrobium officinale culture fluid;

[0035] S2. After inoculating Streptococcus thermophilus in the MRS medium and activating it for 12 hours, inoculate it in the Dendrobium officinale culture solution obtained in step S1, the inoculum size is 1%, at 30° C., the rotation speed is 200 rpm, and the aeration volume is 0vvm. Stirring a...

Embodiment 2

[0037] Embodiment 2 A kind of preparation method of dendrobium officinale extract

[0038] The preparation method of described Dendrobium officinale extract comprises the following steps:

[0039] S1. Beat the dried strips of Dendrobium candidum into small sections with a length less than 2cm, then put the small sections of Dendrobium candidum into an oven and dry them at 65°C for 3 hours, add them into an ultrafine pulverizer and crush them into powder, and sieve them through a 100-mesh sieve. Gained Dendrobium officinale powder is added in the fermentation tank (50L) that 35L water is housed by 50g / L, adds 5% sucrose again, sterilizes, obtains Dendrobium officinale culture fluid;

[0040] S2. After inoculating Streptococcus thermophilus in the MRS medium and activating it for 48h, inoculate it in the Dendrobium officinale culture solution obtained in step S1, the inoculum size is 5%, at 40°C, the rotation speed is 200rpm, and the aeration volume is 0vvm. Stirring and culturi...

Embodiment 3

[0042] Embodiment 3 A kind of preparation method of dendrobium officinale extract

[0043] The preparation method of described Dendrobium officinale extract comprises the following steps:

[0044] S1. Beat the dried strips of Dendrobium candidum into small sections with a length less than 2cm, then put the small sections of Dendrobium candidum into an oven and dry them at 65°C for 3 hours, add them into an ultrafine pulverizer and crush them into powder, and sieve them through a 100-mesh sieve. Gained Dendrobium officinale powder is added in 35L water fermenter (50L) by 30g / L, adds 2% sucrose again, sterilizes, obtains Dendrobium officinale nutrient solution;

[0045] S2. After inoculating Streptococcus thermophilus in the MRS medium and activating it for 24 hours, inoculate it in the Dendrobium officinale culture solution obtained in step S1, the inoculum size is 4%, at 37 ° C, the rotation speed is 200 rpm, and the aeration volume is 0 vvm. Stirring and culturing for 24 hour...

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Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a preparation method of a dendrobium officinale extract. Through double-bacterium secondary fermentation, the method comprises the following steps: firstly using enzymes generated in the lactic acid bacterium fermentation process for decomposing cellulose and cracking plant cell walls to release active substances, then inoculating with saccharomycetes, taking nutrient elements or macromolecules in plant cells as substrates, and decomposing and synthesizing polyphenol flavone and other functional substances beneficial to skin care. The contents of effective components such as phenols, polysaccharides and amino acids can be remarkably increased, and the obtained dendrobium officinale extract has relativelyhigh antioxidant and moisturizing effects; and the method is short in process, simple to operate, high in yield and suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of cosmetics. More specifically, it relates to a preparation method of Dendrobium officinale extract. Background technique [0002] Dendrobium candidum, also known as "black knot grass", is a plant of the genus Dendrobium orchidaceae, and is a perennial epiphytic herb. Since ancient times, Dendrobium officinale has been a precious Chinese herbal medicine in my country. It has the reputation of "gold in medicine" and is also listed as the first of the "Nine Immortal Herbs" by Dao Zang. Dendrobium officinale contains polysaccharides, flavonoids, phenols, amino acids, vitamins and other active ingredients, which have anti-oxidation, anti-aging and treatment of certain diseases, and are often used as functional ingredients in medicines, health products, and cosmetics. Among them, in the "Catalogue of Used Cosmetic Raw Materials (2015 Edition)" issued by the State Food and Drug Administration, the extract of De...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9794A61Q19/08A61Q19/00
CPCA61K8/9794A61Q19/08A61Q19/00A61K2800/85
Inventor 黄小龙张娇韩志东
Owner 泉后(广州)生物科技研究院有限公司
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