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Three-dimensional cell spheroid with high proliferation activity, and producing method and use therefor

A cell, 3D technology, applied in artificial cell constructs, bone/connective tissue cells, biochemical equipment and methods, etc., can solve the problems of destroying extracellular matrix and affecting the expression of proliferative active biological factors, etc.

Pending Publication Date: 2021-03-05
METATECH (AP) INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, enzymes can destroy the extracellular matrix, so after the cells are injected into the organism, their proliferative activity and the expression of biological factors will be affected

Method used

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  • Three-dimensional cell spheroid with high proliferation activity, and producing method and use therefor
  • Three-dimensional cell spheroid with high proliferation activity, and producing method and use therefor
  • Three-dimensional cell spheroid with high proliferation activity, and producing method and use therefor

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Effect test

example 1

[0030] According to the literature J Pharm Pharmacol.2015May;67(5):640-50, fibroblasts need a 3-day recovery period after passing through the needle, so the growth rate of the above-mentioned cell spheroids on the 3rd day after culture is used as a benchmark. Such as figure 2 As shown, when the cell spheroids of the control example were obtained through a needle with a larger inner diameter, the relative growth rate was lower; however, when the cell spheroids of the experimental example were obtained through a needle numbered 30G, the relative growth rate was the lowest. It is the second lowest when obtained through the needle numbered 21G, and the highest when obtained through the needle numbered 27G.

[0031]

[0032] After the above-mentioned cell spheroids were cultured for 3 days, they were washed with PBS first, and then serum-free culture medium was added to continue culturing for 24 hours. Finally, the supernatant was collected for cytokine array analysis to compare ...

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Abstract

The present invention discloses a three-dimensional cell spheroid with high proliferation activity, and the cell spheroid is obtained by ejecting a cell aggregate through a needle with a needle gaugeof less than 30G. The present invention further provides the producing method and the use for the cell spheroid. The 3D structure cell spheres have high proliferative activity and high expression quantity of specific biological factors and are free of carcinogenesis doubts, so that the 3D structure cell spheres can be implanted into a living body, grow rapidly and have biological activity.

Description

technical field [0001] The present invention relates to a cell spheroid, its manufacturing method and application, in particular to a highly proliferative 3D structural cell spheroid, its manufacturing method and application. Background technique [0002] The traditional cell therapy method is to use enzymes to separate single cells from the culture plate, then fill the single cells into a syringe and inject them into the organism through a needle. However, enzymes can destroy the extracellular matrix, so after the cells are injected into the organism, their proliferative activity and the expression of biological factors will be affected. [0003] Therefore, how to improve the traditional cell therapy method is indeed one of the problems actively solved by those skilled in the art. Contents of the invention [0004] The present invention is based on an unexpected discovery, in which a cell aggregate formed by a plurality of cells passing through a needle with a specific i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071A61K35/12A61P35/00A61P9/14A61P17/02A61P43/00A61P19/02A61P19/04A61P9/10A61P25/28A61L27/38A61L27/50
CPCC12N5/0656C12N5/0602A61K35/12A61K9/0019A61P35/00A61P9/14A61P17/02A61P43/00A61P19/02A61P19/04A61P9/10A61P25/28A61L27/3804A61L27/50A61L2400/06C12N5/0062A61K35/33C12N2535/00C12N2513/00
Inventor 陈宗基杨智惠刘恒宇陈彦钧丁侨萱
Owner METATECH (AP) INC
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