Purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity

A technology of proliferation activity and purification method, which is applied in the field of purification of chlorella polysaccharides, and can solve the problems that active components need to be further separated

Active Publication Date: 2021-03-09
NINGXIA MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application provides a purification method of chlorella polysaccharides with anti-proliferation activity of liver cancer cells to solve the problem that the specific active components in chlorella crude polysaccharides need to be further separated

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity
  • Purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity
  • Purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] 1. Preparation of Chlorella Polysaccharides

[0053] 1.1 Cultivation of Autotrophic Chlorella

[0054] The autotrophic Chlorella population was obtained from the Liaoning Academy of Marine and Fishery Sciences. The stock was transferred to 10 L of thrice boiled seawater and grown in F / 2 Mediam (12 hr dark: 12 hr light cycle).

[0055] Autotrophic Chlorella growth conditions: F / 2 (Guillard.1962) modified formula (sea water)

[0056]

[0057]

[0058] The growth cycle of autotrophic Chlorella: Lag period: 0-2d; Growth period: 2-8d; Death period: 8-11d.

[0059] Dry matter components in autotrophic Chlorella: protein: 55-67%; crude fiber: 1-4%; lipid: 8-13%; ash: 5-8%; sugar: 10-20%; moisture 3 to 5%.

[0060] Chlorella polysaccharide components: Chlorella polysaccharides contain the following known sugar groups and active monosaccharides. The molar ratio of known sugar groups is as follows: D-fucose: D-rhamnose: D-2-glucosamine: D-galactose: D-glucose=1.3:1.0:1...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity, which comprises the following steps: carrying out PCA separation on chlorella crude polysaccharide by combining an ion exchange chromatography method and a phenol sulfuric acid colorimetric method to obtain PCA1, PCA2 and PCA3 crude polysaccharide extract, purifying the extractby combining a molecular exclusion chromatography method and a phenol sulfuric acid colorimetric method to obtain refined PCA1 and refined PCA2, detecting the anti-proliferation effects of the fine PCA1 and the fine PCA2 on SMMC-7721 by using a tetramethylazazolium salt trace enzyme reaction colorimetric method, and detecting the content of sulfate radicals in the refined PCA1 and the refined PCA2through a barium chloride turbidity method. According to the PCA2 obtained by the purification method provided by the invention, the cell proliferation of the SMMC-7721 is obviously reduced by 75.49%after the human hepatoma carcinoma cell SMMC-7721 is intervened for 24 hours at the concentration of 1g / L.

Description

technical field [0001] The present application relates to the technical field of separation and purification of polysaccharides and detection of biological activity, in particular to a purification method of chlorella polysaccharides with anti-proliferation activity of liver cancer cells. Background technique [0002] Chlorella (CV) has been extensively studied in recent years due to its diverse biological functions. Studies have shown that Chlorella (CV) has immunomodulatory, anticholesterol and antitumor activities. [0003] Polysaccharides, the active constituents of chlorella, have been found to have anti-inflammatory and immunomodulatory effects. Chlorella crude polysaccharide extract can induce apoptosis of human liver cancer cells SMMC-7721 by down-regulating anti-apoptotic proteins and up-regulating apoptosis execution proteins, which provides a new idea for the screening of anti-hepatic cancer drugs. However, the specific components in the chlorella crude polysacc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A61P35/00A61P1/16
CPCC08B37/0003C08B37/006A61P35/00A61P1/16
Inventor 张瑞徐韬钧黄海月彭楠杨建军
Owner NINGXIA MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap