Purification method of chlorella polysaccharide with anti-hepatoma cell proliferation activity
A technology of proliferation activity and purification method, which is applied in the field of purification of chlorella polysaccharides, and can solve the problems that active components need to be further separated
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[0052] 1. Preparation of Chlorella Polysaccharides
[0053] 1.1 Cultivation of Autotrophic Chlorella
[0054] The autotrophic Chlorella population was obtained from the Liaoning Academy of Marine and Fishery Sciences. The stock was transferred to 10 L of thrice boiled seawater and grown in F / 2 Mediam (12 hr dark: 12 hr light cycle).
[0055] Autotrophic Chlorella growth conditions: F / 2 (Guillard.1962) modified formula (sea water)
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[0058] The growth cycle of autotrophic Chlorella: Lag period: 0-2d; Growth period: 2-8d; Death period: 8-11d.
[0059] Dry matter components in autotrophic Chlorella: protein: 55-67%; crude fiber: 1-4%; lipid: 8-13%; ash: 5-8%; sugar: 10-20%; moisture 3 to 5%.
[0060] Chlorella polysaccharide components: Chlorella polysaccharides contain the following known sugar groups and active monosaccharides. The molar ratio of known sugar groups is as follows: D-fucose: D-rhamnose: D-2-glucosamine: D-galactose: D-glucose=1.3:1.0:1...
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