Perparation method of microencapsulation fetal liver cell suspension
A technology of fetal liver cells and liver cells, which is applied in the field of preparation of drugs for treating liver diseases, can solve problems such as poor proliferation, immune rejection, and patients' inability to tolerate surgery, and achieve the effect of avoiding immune rejection
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[0006] Mix the PSV plasmid with the SV40 large T gene fragment solution, add a T4DNAligase to link it into the PRSV40 plasmid, and then add lipofectimin liposomes to transfect the plasmid into pre-cultivated fetal liver cells; use sodium alginate and polymer Lysine is made into a microencapsulated coating. Place it in the microcapsule generator, and the negative pressure generated by the microcapsule generator will disperse the fetal liver cells transfected with the PRSV40 plasmid in the microcapsule wrapping, forming a microencapsulated and genetically modified fetal liver cell suspension.
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