Perparation method of microencapsulation fetal liver cell suspension
A technology of fetal liver cells and liver cells, which is applied in the field of preparation of drugs for treating liver diseases, can solve problems such as poor proliferation, immune rejection, and patients' inability to tolerate surgery, and achieve the effect of avoiding immune rejection
Inactive Publication Date: 2004-02-25
张阳德
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Problems solved by technology
Due to the complex function of the liver, some treatments that use physical and chemical methods to replace liver detoxification cannot correct severe metabolic disorders of liver disease, so liver cell or liver tissue transplantation is required
Due to the difficulty in the source of liver donors, and some patients cannot tolerate surgery
Liver cell transplantation is adopted, and autologous liver cells are difficult to obtain and have poor proliferation. Therefore, allogeneic naked cell transplantation is often used, but there is a problem of immune rejection
Method used
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Embodiment Construction
[0006] Mix the PSV plasmid with the SV40 large T gene fragment solution, add a T4DNAligase to link it into the PRSV40 plasmid, and then add lipofectimin liposomes to transfect the plasmid into pre-cultivated fetal liver cells; use sodium alginate and polymer Lysine is made into a microencapsulated coating. Place it in the microcapsule generator, and the negative pressure generated by the microcapsule generator will disperse the fetal liver cells transfected with the PRSV40 plasmid in the microcapsule wrapping, forming a microencapsulated and genetically modified fetal liver cell suspension.
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The preparation of microencapsulated fetal liver cell suspension is characterized by that it uses PSV plasmid, mixes it with SV40 fragment solution, adds a kind of catenating enzyme to make it be catenated into RPVS40 plasmid, then adds the liposome to make said plasmid be transfected into precultured liver, cell; uses a kind of microcapsule envelopment compound made up by using sodium alginate and polylysinal and mixes the above-mentioned both them, and utilizes the action of negative pressure produced by microcapsule generator to make the PRSV40 plasmid transfected into the liver cell be dispersed in microcapsule envelopment compound.
Description
technical field [0001] The invention relates to a preparation method of medical medicine, which belongs to a preparation method of medicine for treating liver diseases. Background technique [0002] Liver disease is a frequently-occurring disease. Due to the complex function of the liver, some treatments that use physical and chemical methods to replace liver detoxification cannot correct severe metabolic disorders of liver disease, so liver cell or liver tissue transplantation is required. Due to the difficulty in obtaining liver donors, and some patients cannot tolerate surgery. With liver cell transplantation, it is difficult to obtain autologous liver cells and their proliferative properties are poor. Therefore, allogeneic naked cell transplantation is often used, but there is a problem of immune rejection. Contents of the invention [0003] The task of the present invention is to solve the problem of immune rejection of allogeneic liver cell transplantation and cell...
Claims
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IPC IPC(8): C12N5/10C12N15/85
Inventor 张阳德
Owner 张阳德
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