Evaluation and analysis method for preventing and controlling fusarium verticillioides of corn by chitin-reinforced trichoderma

A technology of Fusarium verticillium and analysis method, which is applied in the field of evaluation and analysis of chitin-enhanced Trichoderma to prevent and control Fusarium verticillium in corn, can solve the problems of crop yield reduction, quality and decline, and achieve the promotion of corn growth and comprehensive and accurate analysis , Improving the effect of corn growth indicators

Pending Publication Date: 2021-03-05
NANJING SINONG BIO ORGANIC FERTILIZERS INST CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002] As the main economic crop in the world, maize is mainly caused by fusarium seedling blig

Method used

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  • Evaluation and analysis method for preventing and controlling fusarium verticillioides of corn by chitin-reinforced trichoderma
  • Evaluation and analysis method for preventing and controlling fusarium verticillioides of corn by chitin-reinforced trichoderma
  • Evaluation and analysis method for preventing and controlling fusarium verticillioides of corn by chitin-reinforced trichoderma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] In this example, the following materials are selected

[0026] Strains: Trichoderma guizhouense NJAU 4742 and fluorescently labeled Fusarium verticillium mutant strain NJAU 1013 were preserved by Jiangsu Provincial Key Laboratory of High-tech Research on Solid Organic Waste Recycling.

[0027] Maize (Zea.mays), commercialized seeds of Zhengdan 958 (approval number: Jishenyu 200002).

[0028] Trichoderma coating medium: 0.2g MgSO4 7H2O, 0.9g K2HPO4, 1g NH4NO3, 0.15g KCl, 3g glucose, 10mL 1 / 300 Bengal red, 20g agar, make up to 1000mL with distilled water, autoclave at 115℃ for 30min. After the culture medium was cooled to 50°C, 25 mL of 1% chloramphenicol, 3 mL of 3% streptomycin, 0.2 g of pentachloronitrobenzene, 1 mL of triton, 1.2 mL of propamocarb (a pesticide), and 150 ppm of hygromycin were added.

[0029] Reagent test kit:

[0030] DNA extraction kit: DNeasy Plant Mini Kit, QIAGEN;

[0031] Fluorescence quantitative kit: SYBR Premix EX Taq kit, Takara, Japan;

...

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Abstract

The invention belongs to the technical field of plant protection experiments, and particularly relates to an evaluation and analysis method for preventing and controlling fusarium verticillioides of corn by chitin-reinforced trichoderma. The method comprises the following steps: performing inoculation treatment on fusarium verticillioides samples under a sterile condition; performing inoculation treatment on trichoderma and fusarium verticillioides samples under aseptic conditions and trichoderma and fusarium verticillioides samples under natural conditions; quantitatively analyzing the quantity of fungi; performing soil culture of corn seedlings; performing inoculation treatment of fungal spores; and determining and analyzing the enzyme activity of soil. The method has guiding significance in applying chitin and trichoderma to prevent and control corn germs.

Description

technical field [0001] The invention belongs to the technical field of plant protection experiments, and in particular relates to an evaluation and analysis method for chitin-enhanced Trichoderma to prevent and control Fusarium verticillium corn. Background technique [0002] As the main economic crop in the world, corn is mainly caused by fusarium seedling blight, which can cause many infections in the roots and stems of corn, resulting in reduced crop yield and quality. At present, Trichoderma, as a soil resident bacterium, has a significant effect on improving soil texture, promoting crop growth and preventing and controlling microbial diseases. In order to achieve the sustainable development of agriculture, the use of biological agents headed by Trichoderma has been favored by industry experts, and the use of Trichoderma to antagonize diseases has been reported at home and abroad. Good results have been achieved. Chitin (Chitin) is a kind of polymer polysaccharide, whi...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12N1/14A01G7/06A01G13/00A01G22/20C12R1/885C12R1/77
CPCC12Q1/18C12N1/14A01G7/06A01G13/00A01G22/20G01N2333/37Y02A40/10
Inventor 宋克超张建华怀峰
Owner NANJING SINONG BIO ORGANIC FERTILIZERS INST CO LTD
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