Perfusion culture chip and perfusion system

Abstract

The invention provides a perfusion culture chip and a perfusion system. The perfusion culture system comprises an upper sealing plate, a culture filter layer and a lower sealing plate layer, the lowersealing plate is provided with an injection interface and a filter-out interface, a filling channel corresponding to the injection interface penetrates the culture filter layer and communicates withthe upper side wall of a culture chamber in the culture filter layer, and filtering holes in the bottom of the culture chamber correspond to the filtering interfaces in position. According to the present invention, perfusion culture is carried out in a manner of injecting the culture solution from the upper part and filtering out the culture solution from the bottom, so that continuous perfusion culture of microorganisms and cells can be realized.

Description

technical field [0001] The invention belongs to the field of organ and tube chips, and in particular relates to a perfusion culture chip and a perfusion system. Background technique [0002] Traditional microorganism or cell culture is mainly carried out in centrifuge tubes, petri dishes or culture bottles, and a sufficient amount of culture medium is added at a time to achieve a certain growth cycle. The microorganisms or cells cultured by this method will show different growth stages as the culture medium is consumed, and cannot be cultivated for a long time and cannot be used for continuous culture. [0003] In order to realize the small-volume perfusion culture of microorganisms, a miniature culture device has appeared, which can add a part of the culture solution. The publication number is CN210065755U, and the name is "a multi-chamber sub-regional petri dish for biomedical microbiology experiment observation" The disadvantage of the Chinese patent is that continuous p...

AI Technical Summary

Problems solved by technology

The microorganisms or cells cultured by this method will show different growth stages as the culture medium is consumed, and cannot be cultivated for a long time, and cannot be used for continuous culture

[0003] In order to realize the small-volume perfusion culture of microorganisms, a miniature culture device has appeared, which can add a part of the culture solution. The publication number is CN210065755U, and the name is "a multi-chamber sub-regional petri dish for biomedical microbiology experiment observation" The disadvantage of the Chinese patent is that continuous perfusion culture cannot be realized; the publication number is CN110923137A, and the Chinese patent titled "a microfluidic device and culture method for in vitro 3D cell and tissue culture" adds There is an inflow and outflow interface, but there is no filter device, it is impossible to culture suspension cells and semi-adherent cells, and it is impossible to realize online observation; the publication number is CN111548939A, and the Chinese patent titled "An Organoid Perfusion Culture Chip and Its Application Method", It is also impossible to add and extract the culture at any time

Images