Compositions and methods for treating endometriosis

A technology for endometriosis and endometrium, applied in biochemical equipment and methods, biological testing, biomaterial analysis, etc., can solve the problem of undiagnosed, untreated, and high cost of endometriosis And other issues

Pending Publication Date: 2021-03-09
BOARD OF RGT THE UNIV OF TEXAS SYST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Screening for endometriosis is impractical because laparoscopy is an invasive procedure and is costly
As a result, many women with endometriosis go undiagnosed, or are diagnosed incorrectly, and are not treated

Method used

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  • Compositions and methods for treating endometriosis
  • Compositions and methods for treating endometriosis
  • Compositions and methods for treating endometriosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1. Gap junction genes are elevated in endometriosis-derived uterine epithelial cells but decreased in endometriosis-derived stromal cells.

[0105] method

[0106] Obtain endometrial tissue in situ (from the uterus) from women with or without endometriosis.

[0107]The presence or absence of endometriosis was confirmed by laparoscopy. All eutopic endometrial samples were obtained during the proliferative phase of the menstrual cycle from normomenstrual women of reproductive age (30-45 years) who were never treated with hormone drugs. Endometriosis samples were obtained from patients classified as stages I-IV according to the American Society for Reproductive Medicine classification. In the case of normal subjects, endometrial tissue was isolated from women undergoing tubal sterilization who were not taking oral contraceptives and who were laparoscopically proven free of endometriosis, submucosal fibroids or uterine Signs of intimal polyps. Primary endometr...

Embodiment 2

[0122] Example 2. Coupling and invasiveness of endometrial epithelial cells (E) and stromal cells (S) to mesothelial cells (M)

[0123] method

[0124] Homotypic and heterotypic gap junction intercellular communication (GJIC) assays (also known as conjugation assays)

[0125] GJIC was measured using cell-to-cell transfer of the fluorescent dye calcein, which is permeable via gap junctions. Assays were performed in medium supplemented with charcoal-adsorbed FBS (10%). Donor cells were incubated with Calcein AM for 20 minutes at room temperature. Inside the cell, calcein AM is cleaved to calcein by nonspecific esterases, making it impermeable for diffusion through the cell membrane. Recipient cells were grown to confluency. Calcein-labeled donor cells were then dropped (‘dropped’) onto the recipient cell layer, and fluorescence microscopy imaging was used to visualize the transfer of calcein between the donor and recipient cells. For homotypic interactions, endometrial ep...

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Abstract

Methods for the detection and diagnosis of endometriosis are disclosed herein. More specifically, sensitive single-cell expression analysis methods to detect specific gene expression patterns in cellpopulations obtained from endometrial cells are provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit and priority of U.S. Provisional Patent Application No. 62 / 660,641, filed April 20, 2018, which is incorporated by reference in its entirety. technical field [0003] The present invention generally relates to methods of detecting and diagnosing endometriosis. Background technique [0004] Endometriosis is one of the most common gynecological disorders in the United States. It is a painful, often debilitating disease that affects more than 6.5 million women in the United States between the ages of 15 and 44 (Buck Louis et al., Fertil Steril, 96:360-365 (2011)). Endometriosis occurs when endometriosis grows abnormally, most commonly on the ovaries, fallopian tubes, the tissues that hold the uterus in place, and on the outer surfaces of the uterus. Although less common, endometrial growths can also be seen on the vagina, cervix, vulva, bowel, bladder, or rectum. The most common symp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50C12Q1/6809C12Q1/686
CPCG01N33/56966G01N2800/364C12Q1/6883C12Q2600/158
Inventor N·柯玛B·尼科尔森
Owner BOARD OF RGT THE UNIV OF TEXAS SYST
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