Preparation method of cardiomyopathy model of beta1 adrenergic receptor

A technology of adrenaline and cardiomyopathy, applied in the field of cardiomyopathy model preparation, can solve the problems of easy coagulation, bacteria breeding in serum, affecting detection, etc., and achieve the effect of inhibiting the growth of bacteria and preventing serum coagulation

Inactive Publication Date: 2021-03-12
张佩霞
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Problems solved by technology

However, in the process of this preparation method, it is necessary to detect the centrifuged serum, and because the detection time is not fixed, it may cause bacteria to grow in the serum during the intermediate interval, thereby affecting the later detection, and if This serum is stored for too long, and it is also prone to coagulation, which will also affect the later detection

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  • Preparation method of cardiomyopathy model of beta1 adrenergic receptor

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preparation example Construction

[0021] A method for preparing a cardiomyopathy model of β1 adrenergic receptor, comprising the following steps:

[0022] S1. Select 14 healthy adult rabbits weighing more than four catties, use the multi-antigen β1 polypeptide to immunize the rabbits with a conventional micro-multiple immunization method, and divide them into groups according to the amount of antigens injected to obtain the immunized rabbits. ;

[0023] S2. Select immunized rabbits in each group, collect venous blood and put it in a centrifuge tube, place the centrifuge tube in a 37°C oven, transfer it to 4°C for precipitation overnight after 2 hours, and take it out the next morning with 1200rpm Spin centrifuge for 10 minutes, then add sodium citrate and NaN to the serum 3 The final concentration is 0.02%;

[0024] S3. Detecting the β1-AR antibody content in the post-immunization serum, counting the β1-AR antibody content corresponding to the amount of antigen injected for different immunizations, and obtai...

Embodiment

[0029] S1. Select 14 healthy adult rabbits weighing more than four catties, use the multi-antigen β1 polypeptide to immunize the rabbits with a conventional micro-multiple immunization method, and divide them into groups according to the amount of antigens injected to obtain the immunized rabbits. ;

[0030] S2. Select immunized rabbits in each group, collect venous blood and put it in a centrifuge tube, place the centrifuge tube in a 37°C oven, transfer it to 4°C for precipitation overnight after 2 hours, and take it out the next morning with 1200rpm Spin centrifuge for 10 minutes, then add sodium citrate and NaN to the serum 3 The final concentration is 0.02%;

[0031] S3. Detecting the β1-AR antibody content in the post-immunization serum, counting the β1-AR antibody content corresponding to the amount of antigen injected for different immunizations, and obtaining the statistical data of different antigen β1 polypeptide injections and the corresponding β1-AR antibody;

[...

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Abstract

The invention relates to the technical field of cardiomyopathy treatment, in particular to a preparation method of a cardiomyopathy model of a beta1 adrenergic receptor. The preparation method comprises the following steps of S1, selecting 14 healthy adult rabbits with the weight of more than 4 jin, immunizing the rabbits by using multi-antigen beta1 polypeptide and adopting a conventional trace multi-time immunization method, and grouping according to the different amounts of inoculation antigen to obtain immunized rabbits; and S2, selecting the immunized rabbits in each group, collecting venous blood, putting the venous blood into centrifuge tubes, putting the centrifuge tubes into an oven at 37 DEG C, transferring the centrifuge tubes to a place at 4 DEG C after 2 hours, precipitating overnight, taking out the venous blood in the morning of the next day for centrifuging for 10 minutes, and adding sodium citrate and NaN3 into serum until the final concentration is 0.02%. According tothe preparation method, serum coagulation can be prevented, growth of bacteria in the serum can be inhibited, and quality guarantee measures are provided for subsequent detection work.

Description

technical field [0001] The invention relates to the technical field of cardiomyopathy treatment, in particular to a method for preparing a β1 adrenergic receptor cardiomyopathy model. Background technique [0002] Autoimmune mechanisms are not only involved in arrhythmias associated with systemic autoimmune diseases, but also are the main cause of idiopathic arrhythmias; it has been clearly established that anti-Ro / SSA antibodies and fetal and neonatal congenital heart block There is a clear correlation with the occurrence of other specific autoantibodies, while other special autoantibodies only affect the bioelectrical activity of myocardial tissue and cause arrhythmia. [0003] In the field of medical research, through indirect research on animal models, factors that are impossible or difficult to exclude under natural conditions are consciously changed in order to more accurately observe the experimental results of the model and conduct comparative studies with human dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02G01N1/30
CPCA01K67/02G01N1/30
Inventor 张佩霞
Owner 张佩霞
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