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A polysaccharide-based nanoparticle co-loading doxorubicin and indocyanine green, its preparation method and application

An indocyanine green and nanoparticle technology, which is applied in the fields of wave energy or particle radiation treatment materials, medical preparations without active ingredients, and medical preparations containing active ingredients, etc. Nanoparticle leakage and other problems, to achieve the effect of improving tumor treatment effect, simple purification process, and preventing leakage

Active Publication Date: 2022-04-22
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most nanoparticles will leak during drug delivery, and it is difficult to achieve rapid and controlled release of drugs

Method used

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  • A polysaccharide-based nanoparticle co-loading doxorubicin and indocyanine green, its preparation method and application
  • A polysaccharide-based nanoparticle co-loading doxorubicin and indocyanine green, its preparation method and application
  • A polysaccharide-based nanoparticle co-loading doxorubicin and indocyanine green, its preparation method and application

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preparation example Construction

[0039] According to the preparation method of nanoparticles co-loaded with doxorubicin and indocyanine green, firstly, doxorubicin and indocyanine green are respectively loaded on aldehyde-based hyaluronic acid and water-soluble hyaluronic acid through Schiff base bond and electrostatic interaction. On the chitosan, they were mixed in a certain proportion to form nanoparticles loaded with doxorubicin and indocyanine green, and then modified with aldolized hyaluronic acid, water-soluble chitosan and Aldolylation of hyaluronic acid with targeting ligands yields polysaccharide-based nanoparticles co-loaded with doxorubicin and indocyanine green. Specifically include the following steps:

[0040] (1) Preparation of doxorubicin-loaded aldehyde-ylated hyaluronic acid nanoparticles: The aldehyde-ylated hyaluronic acid with an oxidation degree of 40% to 100% or 5% to 20% was synthesized by the sodium periodate oxidation method, wherein Aldehydated hyaluronic acid with a degree of oxi...

Embodiment 1

[0059] (1) Preparation of doxorubicin-loaded aldehyde-ylated hyaluronic acid nanoparticles: The aldehyde-ylated hyaluronic acid (MW=8kDa) with an oxidation degree of 40% was synthesized by the sodium periodate oxidation method. The specific process was as follows: The mass concentration of 1.0% hyaluronic acid aqueous solution was added with sodium periodate in a molar amount of 0.4 times the molar amount of the hyaluronic acid structural unit, and the reaction was carried out in the dark for 8 hours at room temperature, and then 2 mL of ethylene glycol was added to terminate the reaction, and dialysis and purification in deionized water for 48 hours were obtained. Formaldehyde-based hyaluronic acid with a theoretical degree of oxidation of 40%;

[0060] This aldolized hyaluronic acid is prepared into a solution with a concentration of 6 mg / mL, and then doxorubicin with a concentration of 2 mg / mL is added dropwise according to the mass ratio of doxorubicin and aldolized hyaluro...

Embodiment 2

[0076] (1) Preparation of doxorubicin-loaded aldehyde-ylated hyaluronic acid nanoparticles: The aldehyde-ylated hyaluronic acid (MW=8kDa) with an oxidation degree of 40% was synthesized by the sodium periodate oxidation method. The specific process was as follows: The mass concentration of 1.0% hyaluronic acid aqueous solution was added with sodium periodate with 0.4 times the number of moles of hyaluronic acid repeating structural units, and the reaction was carried out in the dark for 24 hours at room temperature, and then 2 mL of ethylene glycol was added to terminate the reaction, and dialysis was carried out in deionized water for purification for 72 hours. To obtain aldehyde-based hyaluronic acid with a theoretical degree of oxidation of 40%;

[0077] This aldolized hyaluronic acid was prepared into a solution with a concentration of 6 mg / mL, and then doxorubicin with a concentration of 2 mg / mL was added dropwise according to the mass ratio of doxorubicin to aldolized hya...

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Abstract

A polysaccharide-based nanoparticle co-loading doxorubicin and indocyanine green and its preparation method and application, the nanoparticle co-loading doxorubicin and indocyanine green is placed in a PBS buffer with a pH value of 6.0-6.4 In the method, under the ultrasonic condition of ice bath, the solution of aldylated hyaluronic acid was added dropwise, after the reaction, it was centrifuged and washed with water, and then resuspended in deionized water. Afterwards, carry out centrifugal washing, resuspend in PBS buffer, and finally dropwise add the aldehylated hyaluronic acid solution with the targeting ligand, react for 0.5 to 24 hours, and then wash with centrifugal water to obtain co-loaded doxorubicin and indocyanine green polysaccharide-based nanoparticles. The nanoparticle prepared by the invention is formed through a self-assembly process, the preparation method is simple, the source of raw materials is abundant, drug loading and nanoparticle formation are realized simultaneously, the nanoparticle has good stability under neutral conditions, and the purification process is simple.

Description

technical field [0001] The invention belongs to the field of biological materials, in particular to a polysaccharide-based nanoparticle co-loaded with doxorubicin and indocyanine green, and a preparation method and application thereof. Background technique [0002] Cancer is a major challenge facing mankind, and chemotherapy is one of the main means of cancer treatment. Doxorubicin is a broad-spectrum antitumor chemotherapeutic drug that kills tumor cells by inserting and inhibiting DNA biosynthesis, and is suitable for a variety of tumor treatments. However, doxorubicin, like other chemotherapeutic drugs, has problems such as large toxic side effects and easy induction of drug resistance in cancer cells, which leads to the failure of cancer treatment. To overcome these deficiencies, combination therapy based on different treatment modalities provides new ideas for cancer treatment. [0003] Light therapy has the advantages of non-invasiveness and remote control of time an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/61A61K47/69A61K41/00A61K31/704A61P35/00
CPCA61K47/6939A61K47/61A61K41/0057A61K41/0052A61K31/704A61P35/00A61K2300/00
Inventor 钱军民王亚萍胥伟军侯光晖
Owner XI AN JIAOTONG UNIV