Euphausia superba metal chelating peptide

A technology of metal chelating peptides and Antarctic krill, applied in the biological field, can solve the problems of food calcium source limitation, affecting calcium absorption, insufficient calcium content, etc., and achieve high metal binding activity and antioxidant activity, high antioxidant activity , the effect of improving the utilization rate

CN112480207AActive Publication Date: 2021-03-12ZHEJIANG OCEAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-03-12

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Abstract

The invention discloses a euphausia superba metal chelating peptide, and relates to the technical field of biology. The euphausia superba metal chelating peptide contains the following peptides: Ala-Lys; Glu-Ala-Arg; Ala-Glu-Ala; Val-Glu-Arg-Gly; Val-Ala-Ser; Gly-Pro-Lys; Ser-Pro; Gly-Pro-Lys-Gly; Ala-Pro-Arg-Gly-His; Gly-Val-Pro-Gly; Leu-Glu-Pro-Gly-Pro; Leu-Glu-Lys-Gly-Ala; Phe-Pro-Pro-Gly-Arg;and Gly-Glu-Pro-Gly. The metal chelating peptide provided by the invention has strong chelating ability to calcium and iron, high oxidation resistance and high stability, and can effectively improve the absorption and utilization of iron in human intestinal tracts.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to an Antarctic krill metal chelating peptide. Background technique

[0002] Antarctic krill is usually the guide giant krill (EupHausia superba), which is a marine plankton that exists in the Antarctic waters. 4~6cm. Antarctic krill is also rich in protein and lives in clusters with a density of 10,000-30,000 fish / m 3 , is one of the largest single species of biological resources on the earth, and is also an important food source for key species maintaining the Antarctic ecosystem and high-trophic level organisms. It is the largest potential animal protein resource pool on the earth and is considered to be an important backup protein resource library. Antarctic krill contains about 77.9% to 83.1% water, 0.4% to 3.6% lipid, 11.9% to 15.4% protein and 2% chitin, and also contains 8 kinds of essential amino acids (EAA) and 10 kinds of Non-essential amino acids (NEAA), so i...

Examples

Embodiment 1

[0044] 1.1 Materials and reagents

[0045] Antarctic krill meal (defatted), provided by Hynixon

[0046] Table 1 Experimental Reagents

[0047] Reagent Manufacturer Trypsin American Gibco Corporation Tris Nanjing Jiancheng Biological Engineering DPPH American Sigma Corporation Sodium chloride (analytical pure) Sinopharm Group Hydrochloric acid (analytical pure) Sinopharm Group DEAE-52 Shanghai McLean Biochemical Technology Co., Ltd. SepHadex G-25 Gel Shanghai Yuanju Biological Engineering Co., Ltd.

[0048] 1.2 Experimental Instruments

[0049] Table 2 Experimental Instruments

[0050]

[0051]

[0052] 1.3 Test method

[0053] 1.3.1 Determination of calcium chelation rate

[0054] The determination method of calcium content refers to the titration method (EDTA method) in GB / T 5009.92-2003 Determination of calcium in food. Among them, the 10g / L sodium cyanide solution was replaced with triethanolamine ...

Embodiment 2

[0074] A preparation of Antarctic krill peptide chelated calcium:

[0075] S1: Get the Antarctic krill metalchelating peptide obtained in Example 1, dissolve it in water (mass volume ratio is 1:10g / mL), add calcium chloride (the quality of Antarctic krill metalchelating peptide and calcium chloride The ratio is 1:5), reacted at 60°C, pH 7, and 160rpm for 50min to obtain A solution;

[0076] S2: Add ethanol to the above solution A, stir evenly, remove unbound calcium ions, centrifuge at 10,000 rpm for 5 minutes, take out the precipitate and vacuum freeze-dry to obtain Antarctic krill peptide chelated calcium. Among them, the pre-cooling temperature of the first stage of vacuum freeze-drying is 40°C, 2h; the temperature of the vacuum section in the second stage is -40°C and the pressure is 0Pa, 2h; the third stage is freeze-drying at 25°C and the pressure is 0Pa, 36h .

[0077] The prepared Antarctic krill peptide chelated calcium was tested by using the test method for calciu...

Embodiment 3

[0079] A preparation of Antarctic krill peptide chelated iron:

[0080] S1: Take the above Antarctic krill metal chelating peptide, dissolve it in water (mass volume ratio is 1:10g / mL), add FeSO 4 4H 2 O (Antarctic krill metallochelating peptide with FeSO 4 4H 2 The mass ratio of O is 1:15), then add Na 2 S 2 o 4To a final concentration of 100mM, to prevent the oxidation of ferrous ions, react at 50°C, pH 7, and 160rpm for 5h to obtain A solution;

[0081] S2: Add ethanol to the above solution A, stir evenly, remove unbound iron ions, centrifuge at 10,000 rpm for 5 minutes, take out the precipitate and vacuum freeze-dry to obtain Antarctic krill peptide chelated calcium. Among them, the pre-cooling temperature of the first stage of vacuum freeze-drying is 40°C, 2h; the temperature of the vacuum section in the second stage is -40°C and the pressure is 0Pa, 2h; the third stage is freeze-drying at 25°C and the pressure is 0Pa, 36h .

[0082] Determination of iron chelatio...