Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene module for efficiently degrading feathers and synthesizing artificial hemoprotein and application

A technology of heme protein and feathers, applied in the field of efficient artificial design of biosynthesis system, can solve problems such as resource waste and environmental pollution sources

Pending Publication Date: 2021-03-16
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These wastes accumulate in large quantities, which not only become a potential source of environmental pollution, but also cause a great waste of resources.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene module for efficiently degrading feathers and synthesizing artificial hemoprotein and application
  • Gene module for efficiently degrading feathers and synthesizing artificial hemoprotein and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 Contains the recombinant engineering strain construction of transformation functional module SyKerMb

[0038] 1. Experimental materials

[0039] Cloning vector pJET: a commercially available product from ThermoFisher;

[0040] Expression plasmid pET-28t: kept in our laboratory;

[0041] Escherichia coli BL21(DE3): It is a commercial product of Beijing Quanshijin Company.

[0042] 2. Experimental method

[0043] 1. Through the synthetic biology design module, the full-length nucleic acid sequence of the functional module SyKerMb was obtained by artificial chemical synthesis. Its size is 1635bp, and the module consists of 544 amino acids. It is cloned on the vector pJET, and the recombinant cloning plasmid pJET-SyKerMb containing the complete functional module SyKerMb is constructed and verified by sequencing; then it is digested by Nde I / Xho I Obtain the functional module SyKerMb containing cohesive ends and the expression vector pET-28t vector containin...

Embodiment 2

[0048] Example 2 Biotransformation detection of highly efficient biosynthetic engineering strains containing module SyKerMb

[0049] 1. Experimental materials

[0050] Recombinant engineering strain: the engineering strain BL21-SyKerMb bacterial strain expressing the SyKerMb functional module obtained in Example 1

[0051] Control strain: the BL21-28 strain containing the empty plasmid described in Example 1.

[0052] 2. Experimental method

[0053] 1. Waste feather degradation experiment

[0054] Rinse the chicken feathers with clean water and dry them for later use. Add 50mL inorganic salt medium with feathers as the only carbon and nitrogen source, and sterilize at high temperature. Inorganic salt medium (m / v): NaCl 0.05%, K 2 HPO 4 0.1%, KH 2 PO 4 0.04%, MgCl 2 ·7H 2 O0.01%, CaCl 2 0.006%, pH7.5.

[0055] The seed liquid of the engineering strain expressing the module SyKerMb was transferred to the inorganic salt medium with feathers as the only carbon sourc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

According to the invention, a functional module SyKerMb capable of efficiently degrading poultry feather waste to synthesize novel hemoprotein is artificially designed and created by utilizing a synthetic biological method. A recombinant vector of the functional module is constructed, and the recombinant vector is assembled and expressed in chassis microbial cell escherichia coli. Experiments prove that an engineering strain integrated with the transformation functional module has the biosynthesis capability of degrading animal waste feathers and efficiently synthesizing artificial hemoprotein, and can be applied to related industrial production of artificial protein preparations, artificial foods in the future and the like.

Description

technical field [0001] The invention belongs to the technical field of synthetic biology biotransformation, and relates to an efficient artificially designed biosynthesis system for degrading animal waste such as feathers and transforming and synthesizing heme protein. Background technique [0002] Livestock waste, such as feathers, fur, hooves, horns, etc., are agricultural organic wastes that could not be used for a long time in the past. These wastes have accumulated in large quantities, not only becoming a potential source of environmental pollution, but also causing a great waste of resources. However, these wastes are rich in protein and amino acids and are potential protein and energy resources. Utilizing modern synthetic biology protein intelligent design methods to create an efficient biotransformation cell factory for animal feather organic waste can accelerate the solution to the problem of large amounts of livestock waste accumulation and realize waste emission ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12N15/70C12N1/21C07K14/00C12P17/18C12R1/19
CPCC07K14/00C12N15/70C12P17/182
Inventor 林敏周正富燕永亮张维王劲陆伟
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com