A collagen microfiber hemostatic material and its preparation method and use method

A technology of hemostatic material and collagen material, applied in application, pharmaceutical formulation, surgery and other directions, can solve the problems of non-dispersibility, large collagen fiber size, collagen material denaturation, etc., to maintain biological activity, fast hemostasis, and good hemostasis effect. Effect

Active Publication Date: 2022-05-27
QUANFENG TECH (SHENZHEN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large size of the collagen fibers in the raw material collagen silk thread, up to centimeters, the collagen fibers will eventually aggregate into agglomerates without dispersibility, and cannot be used for hemostasis on bleeding wounds by spraying
[0005] For the collagen hemostatic powder material, "CN105597144A" relates to a collagen hemostatic powder, the collagen solution is spray-dried to prepare collagen particles with a particle size range of 0.1-10mm, but the spray-drying temperature is 100-130°C, this temperature The collagen material under treatment has been denatured, which has a great impact on the biological activity and hemostatic effect of collagen

Method used

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  • A collagen microfiber hemostatic material and its preparation method and use method
  • A collagen microfiber hemostatic material and its preparation method and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of collagen microfiber hemostatic material, wherein, the following steps are included in sequence:

[0036] (1) High-speed shearing of the cross-linked 5g collagen sheet on a high-speed pulverizer, wherein the rotating speed of the high-speed pulverizer is 30000r / min, and the pulverization time is 30s;

[0037] (2) uniformly dispersing the collagen microfiber raw material in step (1) in 500 g of deionized water to prepare a collagen microfiber suspension;

[0038] (3) filtering the collagen microfiber suspension in step (2) through a 30-150 mesh screen, screening to obtain a collagen microfiber raw material with a length of 100-500um, and dispersing in water to prepare a collagen microfiber suspension;

[0039] (4) adding 50 mg of fibrinogen and 2 mg of calcium chloride to the collagen microfibril suspension in step (3);

[0040] (5) adding 5 mg glutaraldehyde and 10 mg NHS ester to the collagen microfibril suspension in step (4), and the reaction ...

Embodiment 2

[0045] A preparation method of collagen microfiber hemostatic material, wherein, the following steps are included in sequence:

[0046] (1) High-speed shearing of 10g collagen film on a high-speed pulverizer, wherein the rotating speed of the high-speed pulverizer is 20000r / min, and the pulverization time is 20s;

[0047] (2) uniformly dispersing the collagen microfiber raw material in step (1) in 100,000 g of deionized water to prepare a collagen microfiber suspension;

[0048] (3) filtering the collagen microfiber suspension in step (2) through a 16-40 mesh screen, screening to obtain a collagen microfiber raw material with a length of 400-1000um, and dispersing in water to prepare a collagen microfiber suspension;

[0049] (4) adding 0.5 mg prothrombin and 0.01 mg platelet cofactor I to the collagen microfibril suspension in step (3);

[0050] (5) adding 2g ethylene oxide and 1g proanthocyanidin in step (4) collagen microfiber suspension, the reaction time is 60min;

[00...

Embodiment 3

[0055] A preparation method of collagen microfiber hemostatic material, wherein, the following steps are included in sequence:

[0056] (1) High-speed shearing of 2g collagen sponge on a high-speed pulverizer, wherein the rotating speed of the high-speed pulverizer is 60000r / min, and the pulverization time is 10s;

[0057] (2) uniformly dispersing the collagen microfiber raw material in step (1) in 500 g of deionized water to prepare a collagen microfiber suspension;

[0058] (3) filtering the collagen microfiber suspension in step (2) through a 80-1000 mesh screen, screening to obtain a collagen microfiber raw material with a length of 10-200um, and dispersing it in water to prepare a collagen microfiber suspension;

[0059] (4) adding 1 mg of prothrombinase to the collagen microfibril suspension in step (3);

[0060] (5) adding 0.02g carbodiimide and 5g genipin in step (4) collagen microfiber suspension, the reaction time is 120min;

[0061] (6) the collagen microfibril su...

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Abstract

The invention relates to the field of biological materials, in particular to a collagen microfiber hemostatic material, a preparation method and a use method thereof. The solid collagen material undergoes high-speed shearing to obtain collagen microfibers. After dispersing the collagen microfibers in the water phase, the procoagulant active ingredients are modified on the surface of the collagen microfibers through linking molecules. Microfiber hemostatic material. Collagen microfiber materials can be easily and efficiently prepared by high-speed shearing, and the biological activity of collagen materials can be kept as much as possible; the length of microfibers ranges from 10um to 1000um, which has high dispersion and is conducive to spraying treatment. Linker molecules can efficiently and quantitatively load procoagulant active ingredients on the surface of collagen microfibrils to enhance their hemostatic effect. The collagen microfiber hemostatic material prepared by the present invention is used for hemostasis on oozing wounds by spraying, such as hemostasis during brain surgery or liver surgery, and can achieve the effect of non-contact fixed-point hemostasis.

Description

technical field [0001] The present invention relates to the preparation and use method of a hemostatic material, in particular to a collagen microfiber hemostatic material, and a preparation method and use method thereof. Background technique [0002] As a local hemostatic material commonly used in clinical practice, solid collagen material has good hemostatic properties. By combining with platelets, it promotes platelet aggregation, directly activates the intrinsic coagulation pathway, and produces coagulation effect. At the same time, blood cells adhere to form thrombus clots, which effectively prevent blood Punch open wounds. In addition, collagen can promote the formation of granulation tissue and accelerate wound healing. A large number of studies have shown that the hemostatic activity of collagen materials is significantly better than that of other polysaccharide hemostatic materials, and it can also have a strong affinity with cells and growth factors in the process...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/102A61L24/001A61L24/0015A61L2400/04A61L2300/418A61L26/0033A61L26/0066A61L26/0076A61L24/0026
Inventor 杜明春曲词
Owner QUANFENG TECH (SHENZHEN) CO LTD
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