6results about How to "Combine accurately" patented technology

This invention provides a method for screening highly specific nucleic acid aptamers and specific *Lactobacillus helveticus* aptamers, belonging to the field of nucleic acid aptamers. Specifically, the method for screening highly specific nucleic acid aptamers provided by this invention includes the following steps: S1: A second library is obtained after N rounds of forward screening of a first library; S2: A third library is obtained after M rounds of reverse screening of the second library; S3: The third library is obtained after X rounds of forward screening of the third library to obtain selected random single-stranded oligonucleotides, which are then subjected to PCR amplification, purification, cloning, and sequencing; S4: The sequences cloned and sequenced in step S3 are used for affinity and specificity assays to select sequences with high affinity and specificity, thereby obtaining highly specific nucleic acid aptamers. The aptamers provided by this invention have the advantages of high specificity and strong affinity, and the construction method of the aptamers provided by this invention has the advantage of short screening time.

This utility model discloses a reinforced molded corrugated bottom cable tray, including a bracket and a cover plate fixedly connected. The inner surface of the bottom of the bracket is provided with corrugated protrusions, and a partition plate overlaps the top of the corrugated protrusions. A mounting plate is fixedly connected to the bottom of the partition plate, and the mounting plate is detachably connected to the inner surface of the bottom of the bracket via a fixing rod. In this application, an appropriate number of partitions are selected, and the partitions are installed in appropriate positions according to wiring requirements. The mounting plate is engaged with the slot to avoid protrusions. The fixing rod passes through the mounting plate and screws into the corresponding screw hole to fix the mounting plate to the partition. The cover plate is then fixed to the bracket, and the connecting sleeve at the corresponding position engages with the top of the corresponding partition. By using the adjustable horizontal position of the partition, the internal space of the bracket is divided and utilized, improving the flexibility of space utilization. Furthermore, the partition installed between the cover plate and the bracket can increase the overall structural strength of the cable tray.

This invention discloses an anti-Dabie Banda virus Gn protein nanobody, a bispecific antibody, and their applications, belonging to the field of biodetection technology. This invention constructs an alpaca natural nanobody library, using recombinantly expressed Gn protein as the antigen. Through multiple rounds of gradient screening using phage display technology, three nanobodies—G15, G20, and G40—that specifically bind to the Dabie Banda virus Gn protein are obtained. All three antibodies exhibit excellent antigen-binding specificity and virus-neutralizing activity; among them, G40 shows the most prominent neutralizing effect. The bispecific antibody G40-C1q, constructed based on G40, combines Gn protein and human C1q protein, achieving a synergistic effect of antiviral activity and inhibition of inflammatory damage. It can be used for the development of drugs for the prevention and treatment of Dabie Banda virus infection and for high-specificity detection reagents, providing candidate antibody drugs for the treatment of fever with thrombocytopenia syndrome, and providing a reliable tool for virus-related research, disease diagnosis, and clinical translation.

The application discloses a kind of cyclic peptide radioactive tracer targeting tumor HER2, labeled precursor and application, it is related to the field of biological medicine.The labeled precursor is A-B-C structure, A is chelating agent, B is connecting arm, C is cyclic peptide compound, the amino acid sequence of cyclic peptide compound is as shown in SEQ ID NO:1, and its 1st cysteine and 10th cysteine form cyclization structure by disulfide bond;Cyclic peptide radioactive tracer includes nuclide and labeled precursor;The application also provides the application of labeled precursor or cyclic peptide radioactive tracer in preparing labeled diagnostic or therapeutic nuclide or HER2 high expression tumor diagnosis and treatment product.The radioactive tracer prepared in the application has good in-vivo stability, has high binding specificity with tumor HER2, has longer tumor retention time, and is simple to synthesize, and has important significance for promoting the clinical application of HER2 targeted polypeptide in nuclear medicine tumor diagnosis and treatment integration.

The application discloses a kind of micropore pre-reaction and pesticide residue detection equipment of immunochromatography, belong to pesticide detection technical field, including shell and for raw material processing processing mechanism, shell is provided with for the pesticide residue of raw material detection detection mechanism and for the replacement of detected liquid replacement mechanism;The application is by integrating units such as broken jar, reference liquid jar, liquid pump and feeding pump in shell, constructs the automatic sample pretreatment system, when detecting, fruit and vegetable samples are broken in broken jar, equipment is pumped into special reference liquid by pH7.2-7.6 phosphate buffer solution, Tween-20, NaCl and gelatin, the reference liquid not only can efficiently extract nicotinamide pesticide in fruit and vegetable, more importantly, its mild component can effectively eliminate the interference of fruit and vegetable matrix to subsequent immune response, while protecting the biological activity of colloidal gold labeled antibody, lay foundation for subsequent high specificity binding, improve the accuracy and reliability of detection.

This invention relates to a gene editing system and its application for intervening in neuronal cerebrospinal fluid deposition disease (INCL). The gene editing system includes a vector and a SpRY-ABE8e base editor coding sequence and an sgRNA-1 coding sequence carried by the vector. The sgRNA-1 targets the pathogenic c.451C>T mutation site region of the PPT1 gene, and its coding sequence is shown in SEQ ID NO:1. The gene editing system of this invention, through the combination of highly specific sgRNA-1 and a specific gene editor, achieves high recognition accuracy and avoids double-strand breaks during editing, thus reducing the possibility of bystander mutations. This addresses the technical problem in existing gene editing systems used for INCL intervention, which struggle to effectively target and repair the PPT1 gene c.451C>T nonsense mutation site, posing significant safety risks.