Substrate for protein printing

Abstract

The invention relates to a product for protein printing, comprising a substrate (1), a first polymer layer (3), which is nanoscale, anti-adhesive to the proteins and deposited on the substrate (1), and a second layer (2) of a benzophenone, which is deposited on said first layer (3), wherein the second layer (2) is solid and soluble in a solvent and the first layer (3) is insoluble in said solvent.

Description

technical field [0001] This application relates to the general field of grafting proteins on substrates, and more particularly to the grafting of proteins in predetermined patterns on substrates by optical means. Background technique [0002] International application with publication number WO 2016 / 050980 (hereinafter referred to as "Studer publication") relates to a method for microstructured or patterned grafting of proteins onto printed or photochemical substrates, wherein the substrate is covered with Nanoscale (between 1 nm and 20 nm) antifouling layer, ie non-stick to living cells. In particular, non-stick layers of this type are polymer brushes or brush-like polymers, especially brush-like PEG (polyethylene glycol). For the protein non-stick layer intended to come into contact with the protein solution, the solution must be aqueous in this known method, so that the layer is insoluble in water to the extent necessary for its use. Such a layer is also intended to be ...

AI Technical Summary

Problems solved by technology

However, this solution complicates the experimental setup

Therefore, maintaining a constant benzophenone concentration in the droplet in the method published by Studer seems advisable, but difficult

Images